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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Macrophage LMO7 deficiency facilitates inflammatory injury via metabolic-epigenetic reprogrammingActa Pharmaceutica Sinica BDecember 1, 2023Shixin Duan, Xinyi Lou, Shiyi Chen, Hongchao Jiang, Dongxin Chen, Rui Yin, Mengkai Li, Yuseng Gou, Wenjuan Zhao, Lei Sun, et al.MAPK (8690), β-actin (8457S), IκBα (4812), PFKFB3 (D7H4Q), Flag-Tag (14793S), HA-Tag (3724S), Myc-Tag (2272S and 2276S), JMJD3 (#3457) and H3K27me3 (C36B11), were purchased from Cell Signaling Technology. DMEM

(2) Genome wide analysis revealed conserved domains involved in the effector discrimination of bacterial type VI secretion systemCommunications BiologyNovember 24, 2023Caihong Wang, Mingxing Chen, Yuhan Shao, Mengyuan Jiang, Quanjie Li, Lihong Chen, Yun Wu, Shan Cen, Nicholas R. Waterfield, Jian Yang, et al.were separated by SDS-PAGE and analyzed by western blot. Anti-FLAG (MA1-91878; Invitrogen), anti-MYC (#2272; Cell Signaling Technology) and anti-Stag (#8476; Cell Signaling Technology) monoclonal antibody at

(3) Photobodies enable the phase-separation and counterbalance of opposing phytochrome B actions in PIF5 degradation and stabilizationbioRxivNovember 18, 2023Ruth Jean Ae Kim, De Fan, Jiangman He, Keunhwa Kim, Juan Du, Meng ChenGrace Bio-Labs, Bend, OR). Myc-tagged mCherry-PIF5 was detected using a rabbit anti-Myc polyclonal antibody (2272, Cell Signaling Technology, Danvers, MA) as the primary antibody at 1:100 dilution and a donkey anti-rabbit

(4) ZIKV induction of tristetraprolin in endothelial and Sertoli cells post-transcriptionally inhibits IFNβ/λ expression and promotes ZIKV persistencemBioOctober 31, 2023William R. Schutt, Jonas N. Conde, Megan C. Mladinich, Grace E. Himmler, Erich R. MackowTTP 1:250 (Cell Signaling D1I3T), IRF3 1:1,000 (Cell Signaling D83B9), Myc 1:1,000 (Cell Signaling 2272S), KHSRP 1:1,000 (Cell Signaling E2E2U), HuR 1:1,000 (Santa Cruz sc-5261), and GAPDH 1:3,000 (Sigma G9545). Statistical

(5) Human OTUD6B positively regulates type I IFN antiviral innate immune responses by deubiquitinating and stabilizing IRF3mBioOctober 31, 2023Jian Wang, Hui Zheng, Chunsheng Dong, Sidong Xiong(1:1,000; sc-8017; Santa Cruz Biotechnology), Flag (1:2,500; F7425; F1804; Sigma-Aldrich), Myc (1:2,000; 2272S; CST), and GAPDH (1:20,000; G9545; Sigma-Aldrich). The band intensities were quantified by ImageJ software

(6) YTHDF2 facilitates aggresome formation via UPF1 in an m6A-independent mannerNature CommunicationsOctober 6, 2023Hyun Jung Hwang, Tae Lim Park, Hyeong-In Kim, Yeonkyoung Park, Geunhee Kim, Chiyeol Song, Won-Ki Cho, Yoon Ki Kim(DYKDDDDK; 14793, Cell Signaling Technology or A8592, Sigma-Aldrich), Myc (9E10; OP10L, Calbiochem or 2272, Cell Signaling Technology), FTO (ab124892, Abcam), GFP (sc-9996, Santa Cruz Biotechnology), DCTN1 (p150glued;

(7) GelMap: intrinsic calibration and deformation mapping for expansion microscopyNature MethodsOctober 1, 2023Hugo G. J. Damstra, Josiah B. Passmore, Albert K. Serweta, Ioannis Koutlas, Mithila Burute, Frank J. Meye, Anna Akhmanova, Lukas C. Kapiteinanti-laminin (1:100 dilution; Abcam, ab11575), rabbit anti-myc-tag (1:100 dilution; Cell Signaling Technology, 2272), rabbit anti-fibrinogen (1:100 dilution; Abcam, ab34269), rat anti-tubulin YL1/2 (1:200 dilution; Abcam,

(8) An interaction between OTULIN and SCRIB uncovers roles for linear ubiquitination in planar cell polarityDis Model MechAugust 1, 2023Stephanie M. Almeida, Sofiia Ivantsiv, Rieko Niibori, Wade H. Dunham, Brooke A. Green, Liang Zhao, Anne-Claude Gingras, Sabine P. Cordesrat anti-HA (1:200, Sigma-Aldrich, clone 3F10), rabbit anti-myc (1:200, Cell Signaling Technology, 2272), rabbit anti-HOIP (1:200, Abcam, ab125189), rabbit anti-SCRIB (1:200, Abcam, ab36708), mouse anti-SCRIB

(9) Global analysis of putative phospholipases in Plasmodium falciparum reveals an essential role of the phosphoinositide-specific phospholipase C in parasite maturationmBioJuly 25, 2023Paul-Christian Burda, Abhinay Ramaprasad, Sabrina Bielfeld, Emma Pietsch, Anna Woitalla, Christoph Söhnchen, Mehar Nihal Singh, Jan Strauss, Aaron Sait, Lucy M. Collinson, et al.diluted in TBS-T containing 5% milk: rabbit anti-Myc (Cell Signalling Technology, Danvers, MA, USA, #2272, 1:1,000), rat anti-HA mAb 3F10 (Sigma, St. Louis, MO, USA, 1:1,000), and mouse anti-GAPDH (58) (1:2,000).

(10) The Host E3-Ubiquitin Ligase TRIM28 Impedes Viral Protein GP4 Ubiquitination and Promotes PRRSV ReplicationInternational journal of molecular sciencesJune 30, 2023Cui Z, Zhou L, Zhao S, Li W, Li J, Chen J, Zhang Y, Xia P.(15202-1-AP) and HRP-β-actin (HRP-60008) were procured from Proteintech. Antibodies for Flag (14793) and Myc (2272) were procured from CST. The antibody for HA (H3663) was procured from Sigma. FITC-labeled goat anti-rabbit

(11) A malaria parasite phospholipase facilitates efficient asexual blood stage egressPLOS PathogensJune 23, 2023Abhinay Ramaprasad, Paul-Christian Burda, Konstantinos Koussis, James A. Thomas, Emma Pietsch, Enrica Calvani, Steven A. Howell, James I. MacRae, Ambrosius P. Snijders, Tim-Wolf Gilberger, et al.an incubation with 30 μl of primary antibody rabbit anti-Myc (1:1.000, Cell Signalling Technology, #2272) in 3% BSA/PBS in a humid chamber for 1 h at RT. After washing cells 3x with PBS, cells were incubated

(12) TRPV4-Rho GTPase complex structures reveal mechanisms of gating and diseaseNature communicationsJune 23, 2023Kwon DH, Zhang F, McCray BA, Feng S, Kumar M, Sullivan JM, Im W, Sumner CJ, Lee SY.(Wavemetrics). Antibodies and reagents Primary antibodies used were rabbit anti-Myc (Cell Signaling Technology, 2272, used at 1:1000 for western blots), mouse anti-Myc (Cell Signaling Technology, 2276, used at 5 μg/ml

(13) EB1 decoration of microtubule lattice facilitates spindle-kinetochore lateral attachment in Plasmodium male gametogenesisNature CommunicationsMay 19, 2023Shuzhen Yang, Mengya Cai, Junjie Huang, Shengnan Zhang, Xiaoli Mo, Kai Jiang, Huiting Cui, Jing Yuanfor immunoblotting IB, 1:1000 for immunofluorescence IF), rabbit anti-Myc (Cell Signaling Technology, cat#2272S, 1:1000 for IF, 1:1000 for IB). Mouse anti-Myc (Cell Signaling Technology, cat#2276S, 1:1000 for IF,

(14) The ubiquitination status of the glucagon receptor determines signal biasJournal of Biological ChemistryMay 1, 2023Suneet Kaur, Badr Sokrat, Megan E. Capozzi, Kimberley El, Yushi Bai, Aeva Jazic, Bridgette Han, Kaavya Krishna Kumar, David A. D'Alessio, Jonathan E. Campbell, et al.no. 8535), TAB1 (catalog no. 3226), STAMBP (catalog no. 5245), rabbit polyclonal Myc tag (catalog no. 2272), rabbit monoclonal GAPDH (horseradish peroxidase conjugate, catalog no. 3683) from Cell Signaling Technology,

(15) Autonomous Synthesis of Functional, Permanently Phosphorylated Proteins for Defining the Interactome of Monomeric 14-3-3ζACS Central ScienceApril 26, 2023Phillip Zhu, Stanislau Stanisheuski, Rachel Franklin, Amber Vogel, Cat Hoang Vesely, Patrick Reardon, Nikolai N. Sluchanko, Joseph S. Beckman, P. Andrew Karplus, Ryan A. Mehl, et al.as described above. Membranes were probed with either Anti-Myc Antibody (Cell Signaling Technology, 2272S) (1:1000 dilution) or Anti-FLAG M2 Antibody (1:1000 dilution). Membranes were washed and then incubated

(16) Tceal7 Regulates Skeletal Muscle Development through Its Interaction with Cdk1International Journal of Molecular SciencesMarch 27, 2023Zhenzhen Xiong, Mengni Wang, Jianhua Wu, Xiaozhong Shiimmunoprecipitation complex was analyzed by western blot with an anti-Myc antibody with a dilution of 1:1000 (#2272, Cell Signaling Technology, Danvers, MA, USA). 4.4. GST-Pulldown Assays GST, GST-Tceal7 and GST-Tceal7

(17) Inhibition of Rab1B Impairs Trafficking and Maturation of SARS-CoV-2 Spike ProteinVirusesMarch 24, 2023Christopher Veeck, Nadine Biedenkopf, Cornelius Rohde, Stephan Becker, Sandro HalweMyc-tagged Rab1A and Rab1B proteins were stained with a rabbit anti-Myc antibody (Cell Signaling, #2272S, dilution 1:50 for immunofluorescence assays (IFAs) and 1:250 for Western blot). Endogenous tubulin

(18) Novel Variants of SOX4 in Patients with Intellectual DisabilityInternational Journal of Molecular SciencesFebruary 9, 2023Martin Grosse, Alma Kuechler, Tabib Dabir, Stephanie Spranger, Stefanie Beck-Wödl, Miriam Bertrand, Tobias B. Haack, Corinna Grasemann, Eva Manka, Christel Depienne, et al.GAPDH were detected using an antibody against myc-tag (Danvers, MA, USA, Cell Signaling Technology, #2272) or GAPDH (Cell Signaling Technology, #2118), respectively. Sequence comparison of the SOX4 HMG boxes

(19) Disruption of the interaction between mutationally activated Gα(q) and Gβγ attenuates aberrant signalingThe Journal of biological chemistryFebruary 1, 2023Aumiller JL, Wedegaertner PB.Mel202, 92.1, OMM1.3, and UM001 lysates), ERK (Cat # 4696S), pERK (Cat # 9101S), and Myc-tag (Cat # 2272S) antibodies were obtained from Cell Signaling Technologies. The HA-tag antibody 12CA5 was from Covance.

(20) Inhibition of stearoyl-CoA desaturase 1 in the mouse impairs pancreatic islet morphogenesis and promotes loss of β-cell identity and α-cell expansion in the mature pancreasMolecular MetabolismJanuary 1, 2023Aneta M. Dobosz, Justyna Janikiewicz, Ewelina Krogulec, Anna Dziewulska, Anna Ajduk, Marcin Szpila, Hanna Nieznańska, Andrzej A. Szczepankiewicz, Dorota Wypych, Agnieszka DobrzynCell Signaling, Hartsfordshire, UK), FOXO1 (catalog no. 2880, Cell Signaling), anti-Myc Taq (catalog no. 2272, Cell Signaling), NKX2.2 (catalog no. sc-398951, Santa Cruz Biotechnology, Santa Cruz, CA, USA), ISL1

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9/1/2016Myc-Tag Antibody to Detect the Expression of Myc -Tagged Proteins Ramanath HegdeDetection of proteins which have no available antibody are made easy by epitope tags. These tags allow the labeling and detection of proteins using immunoblotting, immunoprecipitation, and ... Read Review

Myc-Tag Antibody

Myc-Tag Antibody from Cell Signaling Technology

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Cell Signaling Technology

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Myc-Tag Antibody from Cell Signaling Technology

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