Pacific Blue(TM) anti-mouse/human Helios 137220 from BioLegend

Product Specs
ItemPacific Blue(TM) anti-mouse/human Helios
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number137220
HostArmenian Hamster
NCBI Gene AliasesHELIOS, Helios, ZNF1A2, A730095J18Rik, ANF1A2, Znfn1a2, Zfpn1a2, ZNFN1A2
Clone22F6
Quantity100 tests
IsotypeIgG
ApplicationsIntracellular Staining for Flow Cytometry (ICFC)
ReactivityMouse, Human
Conjugate/TagPacific Blue
NCBI Full Gene NameIKAROS family zinc finger 2
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ImmunogenHelios peptide (aa 51-107)
Antibody TypePrimary
ClonalityMonoclonal
TargetHelios

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BioLegend

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Phone: 1-877-246-5343 Toll-Free, USA & Canada
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Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Covalent TCR-peptide-MHC interactions induce T cell activation and redirect T cell fate in the thymusNature CommunicationsAugust 23, 2022Christopher Szeto, Pirooz Zareie, Rushika C. Wirasinha, Justin B. Zhang, Andrea T. Nguyen, Alan Riboldi-Tunnicliffe, Nicole L. La Gruta, Stephanie Gras, Stephen R. DaleyWaltham, MA, Cat. no.00-5523-00), then incubated with anti-Helios-Pacific Blue (BioLegend, Cat. no. 137220). Samples were then processed using the Click-iT® EdU Flow Cytometry Assay Kit (Thermo Fisher Scientific,

(2) Nfkb2 variants reveal a p100-degradation threshold that defines autoimmune susceptibilityThe Journal of experimental medicineFebruary 1, 2021Wirasinha RC, Davies AR, Srivastava M, Sheridan JM, Sng XYX, Delmonte OM, Dobbs K, Loh KL, Miosge LA, Lee CE, et al.00–5523-00; Thermo Fisher Scientific) and then incubated with antibodies specific for Helios (catalog no. 137220 or 137222; BioLegend) or Foxp3 (catalog no. 11–5773-80; Thermo Fisher Scientific). In some experiments,

(3) An Integrated Epigenomic and Transcriptomic Map of Mouse and Human αβ T Cell DevelopmentImmunityDecember 15, 2020Chopp LB, Gopalan V, Ciucci T, Ruchinskas A, Rae Z, Lagarde M, Gao Y, Li C, Bosticardo M, Pala F, et al.22F6),BioLegend,"Cat# 137218, RRID:AB_10660750" Anti-HELIOS-Pacific Blue (Clone 22F6),BioLegend,"Cat# 137220, RRID:AB_10690535" Anti-MHC Class I (H-2Kb)-APC (Clone AF6-88.5.5.3),Thermofisher,"Cat# 17-5958-82,

(4) An Immunologic Mode of Multigenerational Transmission Governs a Gut Treg SetpointCellJune 11, 2020Ramanan D, Sefik E, Galván-Peña S, Wu M, Yang L, Yang Z, Kostic A, Golovkina TV, Kasper DL, Mathis D, et al.

(5) Salt Sensing by Serum/Glucocorticoid-Regulated Kinase 1 Promotes Th17-like Inflammatory Adaptation of Foxp3⁺ Regulatory T CellsCell reportsFebruary 4, 2020Yang YH, Istomine R, Alvarez F, Al-Aubodah TA, Shi XQ, Takano T, Thornton AM, Shevach EM, Zhang J, Piccirillo CA.

(6) Deletion of self-reactive CCR7- thymocytes in the absence of MHC expression on thymic epithelial cellsCell death and differentiationDecember 1, 2019Wirasinha RC, Chan A, Yap JY, Hu DY, Teh CE, Gray DHD, Goodnow CC, Daley SR.Scientiﬁc, Waltham, MA, Cat #00-5523-00), then incubated with antibodies speciﬁc for Helios (BioLegend, Cat #137220 or #137222) and Foxp3 (Thermo Fisher, Cat #11-5773-80). Samples were then processed using the Click-iT®

(7) Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription FactorCell reportsNovember 12, 2019Shen E, Rabe H, Luo L, Wang L, Wang Q, Yin J, Yang X, Liu W, Sido JM, Nakagawa H, et al.,Cell Signaling Technology,Cat# 12186; RRID: AB_2797842 Anti-mouse Helios (22F6),Biolegend,Cat# 137229; 137220; RRID: AB_2561639; RRID: AB_10690535 Anti-mouse Ki-67 (SolA15),eBioscience,Cat# 12569882; RRID: AB_11150954 Anti-mouse

(8) Targeting the mTOR pathway uncouples the efficacy and toxicity of PD-1 blockade in renal transplantationNature communicationsOctober 17, 2019Esfahani K, Al-Aubodah TA, Thebault P, Lapointe R, Hudson M, Johnson NA, Baran D, Bhulaiga N, Takano T, Cailhier JF, et al.

(9) Differential Dynamics of Regulatory T-Cell and Th17 Cell Balance in Mesenteric Lymph Nodes and Blood following Early Antiretroviral Initiation during Acute Simian Immunodeficiency Virus InfectionJournal of virologyOctober 1, 2019Yero A, Farnos O, Rabezanahary H, Racine G, Estaquier J, Jenabian MA.(PD-1)-BV711,EH12.2H7,BioLegend,329928 FoxP3-PE-CF594,236A/E7,BD Horizon,563955 Helios-Pacific Blue,22F6,BioLegend,137220 IFN-γ-PE-cy7,B27,BD Pharmingen,557643 IL-17A-APC,eBio64DEC17,eBioscience,17-7179-41 Monoclonal

(10) Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1Nature communicationsJuly 26, 2019Leclerc M, Voilin E, Gros G, Corgnac S, de Montpréville V, Validire P, Bismuth G, Mami-Chouaib F.

(11) Decreased maternal serum acetate and impaired fetal thymic and regulatory T cell development in preeclampsiaNature communicationsJuly 10, 2019Hu M, Eviston D, Hsu P, Mariño E, Chidgey A, Santner-Nanan B, Wong K, Richards JL, Yap YA, Collier F, et al.560176, dilution 1:40), AF488 anti-Foxp3 (BioLegend, 320212, 1:50), and V450-anti-Helios (Biolegend, 137220, 1.5:100). Antibodies used for the BIS Cohort include: AF488-anti Foxp3 (BD, 560047, 1:20), PE-anti-CD4

(12) Akt-1 and Akt-2 Differentially Regulate the Development of Experimental Autoimmune Encephalomyelitis by Controlling Proliferation of Thymus-Derived Regulatory T CellsJournal of immunology (Baltimore, Md. : 1950)March 1, 2019Ouyang S, Zeng Q, Tang N, Guo H, Tang R, Yin W, Wang A, Tang H, Zhou J, Xie H, et al.

(13) IKZF2 Drives Leukemia Stem Cell Self-Renewal and Inhibits Myeloid DifferentiationCell stem cellJanuary 3, 2019Park SM, Cho H, Thornton AM, Barlowe TS, Chou T, Chhangawala S, Fairchild L, Taggart J, Chow A, Schurer A, et al.

(14) Differential Roles of IL-2 Signaling in Developing versus Mature TregsCell reportsOctober 30, 2018Fan MY, Low JS, Tanimine N, Finn KK, Priyadharshini B, Germana SK, Kaech SM, Turka LA.clone DNT15Ra",eBioscience,Cat# 17-7149-82; RRID:A3_10718543 "anti-Helios, clone 22F6",BioLegend,Cat# 137220; RRID:A3_10690535 "anti-Foxp3, clone FJK-16 s",eBioscience,Cat# 53-5773-82; RRID:A3_763537 "anti-p-STAT5,

(15) IL-2 prevents deletion of developing T-regulatory cells in the thymusCell death and differentiationJune 1, 2017Hu DY, Wirasinha RC, Goodnow CC, Daley SR.eBioscience, Santa Clara, CA, USA), and then incubated with antibodies specific for Helios (BioLegend; Cat #137220), CTLA-4 (eBioscience; Cat #11-5773-82) and Foxp3 (eBioscience; Cat # 11-5773-80). After washing in

(16) Divergent Phenotypes of Human Regulatory T Cells Expressing the Receptors TIGIT and CD226Journal of immunology (Baltimore, Md. : 1950)July 1, 2015Fuhrman CA, Yeh WI, Seay HR, Saikumar Lakshmi P, Chopra G, Zhang L, Perry DJ, McClymont SA, Yadav M, Lopez MC, et al.

(17) Coexpression of TIGIT and FCRL3 identifies Helios+ human memory regulatory T cellsJournal of immunology (Baltimore, Md. : 1950)April 15, 2015Bin Dhuban K, d'Hennezel E, Nashi E, Bar-Or A, Rieder S, Shevach EM, Nagata S, Piccirillo CA.

(18) Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cellsJournal of immunology (Baltimore, Md. : 1950)March 15, 2015Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson MA, Kekäläinen E, Johansson H, Mjösberg J, Westgren M, Lankisch TO, et al.

(19) Musashi2 sustains the mixed-lineage leukemia-driven stem cell regulatory programThe Journal of clinical investigationMarch 2, 2015Park SM, Gönen M, Vu L, Minuesa G, Tivnan P, Barlowe TS, Taggart J, Lu Y, Deering RP, Hacohen N, et al.were washed 3 times with PBS and incubated with MSI2 (ab76148; Abcam) or IKZF2 antibody Pacific Blue (137220; BioLegend) in 2% FBS in PBS for 1 hour at room temperature. For MSI2 staining, cells were washed twice

(20) Murine CD83-positive T cells mediate suppressor functions in vitro and in vivoImmunobiologyFebruary 1, 2015Kreiser S, Eckhardt J, Kuhnt C, Stein M, Krzyzak L, Seitz C, Tucher C, Knippertz I, Becker C, Günther C, et al.

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9/26/2022Excellent Anti-Mouse Helios Elodie MohrIn different healthy and pathological contexts, CD4 T cells differentiate into a range of specialized subsets, including FOXP3+ regulatory T cells expressing different levels of CD25 and transcription... Read Review

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Pacific Blue(TM) anti-mouse/human Helios from BioLegend

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Pacific Blue(TM) anti-mouse/human Helios from BioLegend

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