Fig 1: The characteristics of B lymphocytes between groups C and D. (A) UMAP of the cluster of B lymphocytes among all cells. (B-C) Two B lymphocyte subsets. UMAP of unbiased clustering and cell annotation of the subsets of B lymphocytes in groups C and D (B). UMAP of unbiased clustering of the subsets of B lymphocytes in groups C and D (C). (D) The proportion of the subsets of B lymphocytes in groups C and D. (E) B lymphocytes were labeled by immunohistochemistry of the sections of femur in groups C and D (CD79a; Scale bar, 40 μm or 20 μm). (F) Key marker genes of B lymphocyte subsets. UMAP of key marker genes of the subsets of B lymphocytes, along with the corresponding distribution of expression levels among 12 clusters. Key marker genes included Bank1, CD83 and Ighd. (G) Latent time and RNA velocity of 3B lymphocyte subsets. Latent time and RNA velocity showed the internal clock of B lymphocyte subsets. Different colors of latent time represent different differentiation times, the colder the color, the earlier, the warmer the color, the later; The arrows of RNA velocity represent the different directions of differentiation. (H) GO analysis of Naïve_B. Bubble diagram of upregulated DEGs of Naïve_B enriched in GO analysis. (I) KEGG analysis of Naïve_B. Bubble diagram of upregulated DEGs of Naïve_B enriched in KEGG analysis. (J-K) The top 5 DEGs of B lymphocytes. The top 5 upregulated DEGs between groups C and D (J). The top 5 downregulated DEGs between groups C and D (K).