Fig 1: SCD-Mediated MUFA Biosynthesis during B Cell Activation In Vitro(A) Heatmap of fatty acid (FA) synthesis gene expression in fresh and LPS/IL-4-activated murine B cells.(B) mRNA expression of four Scd isoforms in freshly and activated B cells, extracted from RNA-seq data. ND, not detected.(C) Immunoblot analysis of SCD2 in fresh and activated murine B cells. Right: summary of SCD2 expression.(D) Reverse transcription polymerase chain reaction (RT-PCR) analysis of ACACA, FASN, and SCD expression in human B cells at 0, 8, and 24 h after CpG/anti-CD40/IL-15/IL-10/IL-2 activation.(E) RT-PCR analysis of Acaca, Fasn, Scd1, and Scd2 expression in fresh and activated murine B cells treated with rapamycin (10 nM) or Torin 1 (100 nM).(F) Composition (among all cellular FAs) of palmitoleic acid (PO), oleic acid (OA), palmitic acid (PA), stearic acid (SA), linoleic acid (LA), and arachidonic acid (AA) in fresh and activated murine B cells treated with vehicle or the SCD inhibitor SSI-4 for 48 h (n = 3).(G) Isotopomer distribution of U-13C-glucose incorporation in OA, SA, PO, and PA in activated B cell treated with vehicle or SSI-4 at 48 h (n = 3).(H) Isotopomer distribution of U-13C-glucose incorporation in OA and PO in activated B cells from WT or Cd2iCreScd1fl/flScd2fl/fl mice (n = 3).p values were calculated using Student’s t test (C, G, and H) and one-way ANOVA (E and F). Data are representative of at least three (C–E) or two (F–H) independent experiments. Error bars represent SEM. See also Figure S1.