Fig 2: Downregulation of miR-214-5p enhanced the protein expression of COL4A1, TGF-β and p-Smad2. (A) Western blot analysis demonstrated that miR-214-5p downregulation increased the protein levels of COL4A1, TGF-β and p-Smad2. Densitometric analysis of western blotting results was performed to quantify the protein expression of (B) COL4A1, (C) TGF-β and (D) p-Smad2 following downregulation of miR-214-5p in human bone marrow stem cells. ##P<0.01 vs. negative control group. miR, microRNA; COL4A1, collagen type IV α1 chain; TGF-β, transforming growth factor-β; p-Smad2, phosphorylated-Smad2; negative, negative control transfection group; anti-miR-214-5p group, miR-214-5p downregulation group.

Fig 3: Overexpression of miR-214-5p suppressed COL4A1, TGF-β and p-Smad2 protein levels. (A) Western blot analysis demonstrated that miR-214-5p overexpression reduced the protein levels of COL4A1, TGF-β and p-Smad2. Densitometric analysis of western blotting results was performed to quantify the protein expression of (B) COL4A1, (C) TGF-β and (D) p-Smad2 following overexpression of miR-214-5p in human bone marrow stem cells. ##P<0.01 vs. negative control group. miR, microRNA; COL4A1, collagen type IV α1 chain; TGF-β, transforming growth factor-β; p-Smad2, phosphorylated-Smad2; negative, negative control transfection group; miR-214-5p group, overexpression of miR-214-5p group.