Cas9 (7A9-3A3) Antibody sc-517386 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemCas9 (7A9-3A3) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numbersc-517386
Quantity200 µg/ml
ApplicationsWB, IP, IF, IHC(P), FCM
Conjugate/TagUnconjugated
ReactivityS. pyogenes
TargetCas9
Clone7A9-3A3
Hostmouse
Antibody TypePrimary Antibodies
ClonalityMonoclonal
FormatPurified
IsotypeIgG1

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Germline cis variant determines epigenetic regulation of the anti-cancer drug metabolism gene dihydropyrimidine dehydrogenase (DPYD)bioRxivNovember 9, 2023Ting Zhang, Alisa Ambrodji, Huixing Huang, Kelly J. Bouchonville, Amy S. Etheridge, Remington E. Schmidt, Brianna M. Bembenek, Zoey B. Temesgen, Zhiquan Wang, Federico Innocenti, et al.Thermo Fisher Scientific, Waltham, MA), anti-alpha Tubulin (ab4074; 1:7,500 dilution; Abcam, Waltham, MA), and anti-cas9 (sc-517386; 1:1,000 dilution; Santa Cruz Biotechnology, Dallas, TX). Membranes were washed and incubated with anti-mouse

(2) Specificity of cancer-related chromosomal translocations is linked to proximity after the DNA double-strand break and subsequent selectionNAR CancerSeptember 23, 2023Reynand Jay Canoy, Anna Shmakova, Anna Karpukhina, Nikolai Lomov, Eugenia Tiukacheva, Yana Kozhevnikova, Franck André, Diego Germini, Yegor Vassetzky(32) using the following antibodies: Cas9 antibody (7A9-3A3) (anti-mouse, Santa Cruz Biotechnology, sc-517386), GAPDH (anti-mouse, Cell Signaling Technology, 2118) and anti-mouse peroxidase-conjugated secondary

(3) From a drug repositioning to a structure-based drug design approach to tackle acute lymphoblastic leukemiaNature CommunicationsMay 29, 2023Magali Saez-Ayala, Laurent Hoffer, Sébastien Abel, Khaoula Ben Yaala, Benoit Sicard, Guillaume P. Andrieu, Mehdi Latiri, Emma K. Davison, Marco A. Ciufolini, Paul Brémond, et al.confirmed after doxycycline exposition (1 µg/ml) by western blot with CRISPR-Cas9 antibody (Santa Cruz, SC-517386, 1:1000). Cas9-expressing cell line was further transduced with human DCK sgRNA purified lentiviral

(4) Optimized metrics for orthogonal combinatorial CRISPR screensScientific ReportsMay 6, 2023Ronay Cetin, Martin Wegner, Leah Luwisch, Sarada Saud, Tatjana Achmedov, Sebastian Süsser, Antonella Vera-Guapi, Konstantin Müller, Yves Matthess, Eva Quandt, et al.primary antibodies overnight at 4 °C: 1:1000 mouse monoclonal anti-Cas9 (Santa Cruz Biotechnology, sc-517386) or 1:1000 mouse monoclonal anti-HA (clone 12CA5, Merck KGaA, 11583816001) or 1:1000 rabbit monoclonal

(5) Loss of RREB1 in pancreatic beta cells reduces cellular insulin content and affects endocrine cell gene expressionDiabetologiaApril 1, 2023Katia K. Mattis, Nicole A. J. Krentz, Christoph Metzendorf, Fernando Abaitua, Aliya F. Spigelman, Han Sun, Jennifer M. Ikle, Swaraj Thaman, Antje K. Rottner, Austin Bautista, et al.RRID:AB_10671224), RFX6 (1:1000, cat. no. HPA037696) (all Sigma-Aldrich, UK), Cas9 (1:1000, cat. no. sc-517386, RRID:AB_2800509) or β-tubulin (1:2000, cat. no. sc-365791, RRID:AB_10841919) (both Santa Cruz Biotechnology,

(6) A multi-omic dissection of super-enhancer driven oncogenic gene expression programs in ovarian cancerNature CommunicationsDecember 1, 2022Michael R. Kelly, Kamila Wisniewska, Matthew J. Regner, Michael W. Lewis, Andrea A. Perreault, Eric S. Davis, Douglas H. Phanstiel, Joel S. Parker, Hector L. Franco1:5000 in 5% BSA in TBST and incubated overnight at 4 °C. The Cas9 antibody (7A9-3A3) (Santa Cruz, sc-517386) was diluted at 1:1500 in 5% BSA in TBST and incubated overnight at 4 °C. The secondary antibodies (Donkey anti-rabbit IgG HRP

(7) Low-dose AAV-CRISPR-mediated liver-specific knock-in restored hemostasis in neonatal hemophilia B mice with subtle antibody responseNature CommunicationsNovember 25, 2022Xiangjun He, Zhenjie Zhang, Junyi Xue, Yaofeng Wang, Siqi Zhang, Junkang Wei, Chenzi Zhang, Jue Wang, Brian Anugerah Urip, Chun Christopher Ngan, et al.duplicate. In addition to experimental samples, a monoclonal mouse anti-SpCas9 antibody (Santa Cruz, #sc-517386) was serially diluted and added in duplicate wells to generate the standard curve. The plates were incubated

(8) CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cellsScience advancesJuly 15, 2022Werder RB, Liu T, Abo KM, Lindstrom-Vautrin J, Villacorta-Martin C, Huang J, Hinds A, Boyer N, Bullitt E, Liesa M, et al.20 (TBS-T) and then probed with primary antibody [Cas9 antibody (7A9-3A3), Santa Cruz Biotechnology, sc-517386, or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody, Millipore, MAB374] overnight at 4°C.

(9) Genome-Wide CRISPR Screening Identifies DCK and CCNL1 as Genes That Contribute to Gemcitabine Resistance in Pancreatic CancerCancers (Basel)June 27, 2022Hai Yang et al.RRID: AB_10680904) and Cas9 antibodies from Santa Cruz Biotechnology (Santa Cruz Biotechnology Cat. # sc-517386, RRID: AB_2800509). From Cell Signaling Technology, we purchased GAPDH (Cell Signaling Technology Cat.

(10) Cytosolic microRNA-inducible nuclear translocation of Cas9 protein for disease-specific genome modificationNucleic Acids ResearchJune 10, 2022Cheol-Hee Shin, Su Chan Park, Il-Geun Park, Hyerim Kim, Byoungha An, Choongil Lee, Sang-Heon Kim, Juyong Lee, Ji Min Lee, Seung Ja OhBiosystems 7500 real-time cyclers as described previously (17). Western blot Antibodies specific for Cas9 (sc-517386), His (sc-8036), GFP (SC-9996) were purchased from Santa Cruz Biotechnology and EZH2 (3147), ERK (9102),

(11) Repurposing Itraconazole and Hydroxychloroquine to Target Lysosomal Homeostasis in Epithelial Ovarian CancerCancer Research CommunicationsMay 4, 2022Stefano Marastoni, Ainhoa Madariaga, Aleksandra Pesic, Sree Narayanan Nair, Zhu Juan Li, Zvi Shalev, Troy Ketela, Ilaria Colombo, Victoria Mandilaras, Michael Cabanero, et al.Mycoplasma using the Mycoalert Detection Kit (Lonza). Antibodies, Drugs, and Reagents Anti-Cas9 (sc-517386, RRID:AB_2800509) antibody was purchased from Santa Cruz Biotechnology. Anti-beta Tubulin (ab6046, RRID:AB_2210370)

(12) Enhancer RNA Transcription Is Essential for a Novel CSF1 Enhancer in Triple-Negative Breast CancerCancers (Basel)April 6, 2022Michael W. Lewis, Kamila Wisniewska, Caitlin M. King, Shen Li, Alisha Coffey, Michael R. Kelly, Matthew J. Regner, Hector L. FrancoAnti-beta Tubulin Loading Control (Abcam, ab6046), Anti-Cas9 Antibody (7A9-3A3) (Santa Cruz Biotechnology, sc-517386). β-tubulin antibody was used at a 1:1500 dilution in 5% BSA in TBST with overnight incubation at 4

(13) Streptococcal pyrogenic exotoxin B cleaves GSDMA and triggers pyroptosisNatureFebruary 1, 2022Deng W, Bai Y, Deng F, Pan Y, Mei S, Zheng Z, Min R, Wu Z, Li W, Miao R, et al.polyclonal anti-His (Cell Signaling Technology, 2365), mouse monoclonal anti-Cas9 (Santa Cruz Biotechnology, sc-517386), mouse monoclonal anti-GSDMD (Santa Cruz Biotechnology, sc-81868), rabbit lgG control antibody (Vector Laboratories

(14) ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neuronsThe Journal of experimental medicineDecember 6, 2021Ma L, Yu L, Jiang BC, Wang J, Guo X, Huang Y, Ren J, Sun N, Gao DS, Ding H, et al.Proteintech; 66009–1-1G), mouse anti-GFAP (1:2,000; CST; 3670S), mouse anti-CAS9 (1:200; Santa Cruz; sc-517386), rabbit anti-ZNF382 (1:1,500; Abcam; ab51276), rabbit anti-GAPDH (1:5,000; Santa Cruz; sc-25778), rabbit

(15) Computational inference of cancer-specific vulnerabilities in clinical samplesGenome biologyJune 29, 2020Jang K, Park MJ, Park JS, Hwangbo H, Sung MK, Kim S, Jung J, Lee JW, Ahn SH, Chang S, et al.proteins were used per lane. The blot was probed with anti-Cas9 (1:1000; Santa Cruz Biotechnology, sc-517386), anti-PSMB4 (1:500; Santa Cruz Biotechnology, sc-390878), anti-Bad (1:1000; Cell Signaling Technology,

(16) A CRISPR/Cas9 genome editing pipeline in the EndoC-Î²H1 cell line to study genes implicated in beta cell functionWellcome Open ResearchApril 29, 2020Antje K. Grotz, Fernando Abaitua, Elena Navarro-Guerrero, Benoit Hastoy, Daniel Ebner, Anna L. Gloynmonoclonal AB_2800508 NEUROD1 Santa Cruz, sc-46684 1:1000 Mouse monoclonal AB_671759 Cas9 Santa Cruz, sc-517386 1:1000 Mouse monoclonal AB_2800509 CHOP Abcam, ab179823 1:1000 Rabbit monoclonal AB_10703186 pPERK Cell Signaling

(17) Leishmania donovani parasite requires Atg8 protein for infectivity and survival under stressCell death & diseaseOctober 24, 2019Giri S, Shaha C.after densitometry of the blots. Anti-Cas9 antibody was obtained from Santa Cruz Biotechnology, Inc. (sc-517386, Dallas, TX). Autophagy measurement The level of autophagy in the parasites was measured by staining

(18) A CRISPR/Cas9 genome editing pipeline in the EndoC-βH1 cell line to study genes implicated in beta cell functionWellcome Open ResearchOctober 8, 2019Antje K. Grotz, Fernando Abaitua, Elena Navarro-Guerrero, Benoit Hastoy, Daniel Ebner, Anna L. Gloynmonoclonal AB_2800508 NEUROD1 Santa Cruz, sc-46684 1:1000 Mouse monoclonal AB_671759 Cas9 Santa Cruz, sc-517386 1:1000 Mouse monoclonal AB_2800509 CHOP Abcam, ab179823 1:1000 Rabbit monoclonal AB_10703186 pPERK Cell Signaling

(19) Chemogenetic control of gene expression and cell signaling with antiviral drugsNature MethodsJuly 2, 2018Elliot P. Tague, Hannah L. Dotson, Shannon N. Tunney, D. Christopher Sloas, John T. NgoCorning). Antibodies The following primary antibodies were used: mouse anti-Cas9 (Santa Cruz Biotechnology, sc-517386, 1:500 dilution for western blotting, 1:50 for immunostaining), mouse anti-human CD184 (CXCR-4) APC

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Cas9 (7A9-3A3) Antibody from Santa Cruz Biotechnology, Inc.

Santa Cruz Biotechnology, Inc.

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Cas9 (7A9-3A3) Antibody from Santa Cruz Biotechnology, Inc.

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