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Antibodies
Anti-POLR3A antibody
Anti-POLR3A antibody from Abcam
Be the first to write a review!
Citations:
(14)
Supplier Page
Supplier Page from
Abcam for
Anti-POLR3A antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-POLR3A antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab96328
Quantity
100 UL
Conjugate/Tag
Unconjugated
Clonality
Polyclonal
Applications
Western blot
Isotype
IgG
Host
Rabbit
Antibody Type
Primary
Concentration
0.89 mg/ml
Gene Name
POLR3A
Reactivity
Human
Target
POLR3A
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (14)
(1)
Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture
Genome biology
November 28, 2022
Jiang Y, Huang J, Tian K, Yi X, Zheng H, Zhu Y, Guo T, Ji X.
Sc-48385
Anti-RNA Pol II-CTD,
Abcam
,Cat #
ab817
Anti-RNA Pol II-NTD,CST,Cat # D8L4Y Anti-POLR3A,
Abcam
,Cat #
ab96328
Anti-GFP,
Abcam
,Cat #
ab290
Anti-HA tag,
Abcam
,Cat #
ab9110
Anti-β-Actin,
Sigma-Aldrich
,Cat #
A2228
Anti-RPAC1,
Santa Cruz
(2)
A cancer-associated RNA polymerase III identity drives robust transcription and expression of snaR-A noncoding RNA
Nature communications
May 30, 2022
Van Bortle K, Marciano DP, Liu Q, Chou T, Lipchik AM, Gollapudi S, Geller BS, Monte E, Kamakaka RT, Snyder MP.
(Catalog#
34096
,
Thermo Scientific
). Antibodies used
for immunoblotting
include Rabbit Anti-POLR3A (
Abcam
,
ab96328
lot#GR318563) [
1:1,000
], Rabbit Anti-
POLR3B
(
Bethyl
,
A301-855A
) [
1:1,000
], Rabbit Anti-
POLR3C
(
Bethyl
,
(3)
5′ Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos
Science Advances
November 19, 2021
Luxi Chen et al.
8.0), 167 mM NaCl, and 1× protease
inhibitor
cocktail]. Ten micrograms of anti-POLR3A antibody (
Abcam
,
ab96328
) or immunoglobulin G (IgG) (
Cell Signaling Technology
,
2729S
) was added to the lysate, followed by
incubation
(4)
Alteration of the Premature tRNA Landscape by Gammaherpesvirus Infection
mBio
December 22, 2020
Jessica M. Tucker, Aaron M. Schaller, Ian Willis, Britt A. Glaunsinger
discarded. Forty micrograms of
chromatin
was incubated with 10 μg rabbit polyclonal anti-POLR3A (
Abcam
ab96328
) or rabbit IgG (Southern Biotech) overnight. Thirty microliters of mixed
protein A
and G Dynabeads (
Thermo Fisher
(5)
Structure of human RNA polymerase III
Nature Communications
December 1, 2020
Ewan Phillip Ramsay et al.
per the manufacturers’ instructions. Antibodies The following primary antibodies were used: POLR3A (
ab96328
,
Abcam
),
POLR3B
(
ab137030
,
Abcam
), POLR3D (
ab86786
,
Abcam
),
POLR3E
(
ab134560
,
Abcam
), HA-tag (
ab9110
,
(6)
APOE4 exacerbates synapse loss and neurodegeneration in Alzheimer's disease patient iPSC-derived cerebral organoids
Nature communications
November 2, 2020
Zhao J, Fu Y, Yamazaki Y, Ren Y, Davis MD, Liu CC, Lu W, Wang X, Chen K, Cherukuri Y,
et al
.
(
Cell Signaling Technology
,
9091
,
1:1000
),
G3BP
(BD,
611126
,
1:1000
), ERCC4 (
Fitzgerald
,
10R-4026
,
1:1000
), POLR3A (
Abcam
,
ab96328
,
1:1000
), and
HSPA4
(
Cell Signaling Technology
,
3303
,
1:1000
). All uncropped blot images with molecular
(7)
BRAF inhibition sensitizes melanoma cells to α-amanitin via decreased RNA polymerase II assembly
Scientific reports
May 23, 2019
Frischknecht L, Britschgi C, Galliker P, Christinat Y, Vichalkovski A, Gstaiger M, Kovacs WJ, Krek W.
used in a
1:1000
dilution. Alpha-tubulin (
ab18251
) (
1:2000
), POLR1A (
ab139130
) (
1:500
) and POLR3A (
ab96328
) (
1:500
) were purchased from
Abcam
, PARP (
556362
) (
1:1000
) and p53 (
554293
) (
1:1000
) from
BD
Pharmingen,
(8)
Leukodystrophy-associated
POLR3A
mutations down-regulate the RNA polymerase III transcript and important regulatory RNA
BC200
The Journal of biological chemistry
May 3, 2019
Choquet K, Forget D, Meloche E, Dicaire MJ, Bernard G, Vanderver A, Schiffmann R, Fabian MR, Teichmann M, Coulombe B,
et al
.
transferred onto a nitrocellulose
membrane
(Bio-Rad).
Immunoblots
were probed with anti-POLR3A (
Abcam
no.
ab96328
), anti-
GAPDH
(
GeneTex
no.
GTX627408
), and anti-actin (
Abcam
no.
ab3280
). Small RNA and tRNA precursor
(9)
Changes in mRNA abundance drive shuttling of RNA binding proteins, linking cytoplasmic RNA degradation to transcription
eLife
October 3, 2018
Gilbertson S, Federspiel JD, Hartenian E, Cristea IM, Glaunsinger B.
(
Abcam
ab5095
), rabbit polyclonal anti-TBP (
Abcam
ab28175
), or rabbit polyclonal anti-POLR3A (
Abcam
ab96328
) overnight, whereupon samples were rotated with 20 μl protein G dynabeads (with
mouse
antibodies), or
(10)
Epigenetic regulation of RNA polymerase III transcription in early breast tumorigenesis
Oncogene
December 7, 2017
J-L Park et al.
X-100, 280 mM NaCl, 0.2% deoxycholate) and
immunoprecipitated
with antibodies against POLR3A (cat #
ab96328
from
Abcam
, Cambridge, MA, USA),
MYC
(#
9402
from
Cell Signaling Technology
, Danvers, MA, USA), H3K4me3
(11)
Genome-wide mapping of infection-induced SINE RNAs reveals a role in selective mRNA export
Nucleic Acids Research
June 2, 2017
John Karijolich, Yang Zhao, Ravi Alla, Britt Glaunsinger
for
chromatin
shearing (50). Cross-linked
chromatin
was
immunoprecipitated
using anti-POLR3A (
clone
ab96328
;
Abcam
) and
normal rabbit IgG
(
clone
2729
;
Cell Signaling
). Following reversal of cross-links, DNA was
(12)
Absence of neurological abnormalities in mice homozygous for the Polr3a G672E hypomyelinating leukodystrophy mutation
Molecular brain
April 13, 2017
Choquet K, Yang S, Moir RD, Forget D, Larivière R, Bouchard A, Poitras C, Sgarioto N, Dicaire MJ, Noohi F,
et al
.
antibodies. For measurement of POLR3A protein levels,
immunoblots
were probed with anti-POLR3A (
Abcam
#
ab96328
) and anti-actin (
Abcam
#
ab3280
). RNA extraction and
Northern Blots
Cerebral hemispheres and livers
(13)
MAF1 represses CDKN1A through a Pol III-dependent mechanism
eLife
June 12, 2015
Lee YL, Li YC, Su CH, Chiao CH, Lin IH, Hsu MT.
transferred onto a nitrocellulose
membrane
. The antibody dilutions used were rabbit anti-POLR3A (
1:1000
;
ab96328
,
Abcam
; Cambridge, England), rabbit anti-MAF1 (
1:1000
; GTX106776, Acris; Herford, Germany), rabbit anti-CDKN1A
(14)
A Novel Monoclonal Antibody to Secreted Frizzled-Related Protein 2 Inhibits Tumor Growth
Molecular Cancer Therapeutics
May 1, 2013
Emily Fontenot et al.
The nuclear loading control of
TATA binding protein TBP antibody
(
ab63766
), NFAT4 (which is NFATc3) (
ab96328
) and Ki-67 were purchased from
Abcam
, Inc. (Cambridge, MA). CD31 primary antibody was purchased from
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Reviews
Be the First to Write a Review!
Anti-POLR3A antibody from Abcam
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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