Goat Anti-Mouse IgG2a heavy chain (HRP) ab97245 from Abcam

Product Specs
ItemGoat Anti-Mouse IgG2a heavy chain (HRP)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab97245
Quantity1 mg
ApplicationsELISA, Immunocytochemistry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Antibody TypeSecondary
IsotypeIgG
HostGoat
Conjugate/TagHRP
FormatAntiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP)
Concentration1 mg/ml
ClonalityPolyclonal
TargetIgG2a

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(1) A Pvs25 mRNA vaccine induces complete and durable transmission-blocking immunity to Plasmodium vivaxNPJ VaccinesDecember 14, 2023Nawapol Kunkeaw, Wang Nguitragool, Eizo Takashima, Niwat Kangwanrangsan, Hiromi Muramatsu, Mayumi Tachibana, Tomoko Ishino, Paulo J. C. Lin, Ying K. Tam, Sathit Pichyangkul, et al.determination of antibody titers, except that HRP-conjugated anti-mouse IgG1 (Abcam ab97240) or IgG2a (Abcam ab97245) was applied instead. Avidity ELISA: the diluted sera were assayed in the presence and absence of 1

(2) Excretory/Secretory Products from Schistosoma japonicum Eggs Alleviate Ovalbumin-Induced Allergic Airway InflammationPLOS Neglected Tropical DiseasesOctober 3, 2023Zhidan Li, Xiaoling Wang, Wei Zhang, Wenbin Yang, Bin Xu, Wei HuSubsequently, 100 μL of HRP-labeled goat anti-mouse IgE, IgG1 or IgG2a (Cat# ab99574, ab97240, and ab97245; 1: 2000; Abcam, UK) antibodies previously diluted with PBST were added to each well. After incubation

(3) Modifying immune responses to adeno-associated virus vectors by capsid engineeringMolecular Therapy - Methods & Clinical DevelopmentSeptember 14, 2023Martin Bentler, Romain Hardet, Moritz Ertelt, Daniela Rudolf, Dorota Kaniowska, Andreas Schneider, Florian W.R. Vondran, Clara T. Schoeder, Marion Delphin, Julie Lucifora, et al.bound to coated AAV2 capsids were captured with an anti-mouse, HRP-coupled IgG2a antibody (1:25,000, ab97245, Abcam). Antibody complexes were visualized by addition of TMB (Sigma-Aldrich) and subsequently sulfuric

(4) PLGA Nanoparticle-Based Dissolving Microneedle Vaccine of Clostridium perfringens ε ToxinToxins (Basel)July 19, 2023Wei Wan, Yue Li, Jing Wang, Zhiying Jin, Wenwen Xin, Lin Kang, Junhong Wang, Xiaoyang Li, Yakun Cao, Hao Yang, et al.ETX-specific antibodies IgG (Solarbio C1055, Beijing, China), IgG1 (ab97240, Abcam, UK), and IgG2a (ab97245, Abcam, UK) were measured by indirect ELISA. In brief, rETXY196E-C protein was diluted to 10 μg/mL with

(5) A mosquito AgTRIO mRNA vaccine contributes to immunity against malarianpj VaccinesJune 7, 2023Yu-Min Chuang, Mohamad-Gabriel Alameh, Selma Abouneameh, Hamidah Raduwan, Michel Ledizet, Drew Weissman, Erol Fikrigperoxidase-conjugated goat anti-mouse IgG1, IgG2a, IgG2b, or IgG3 heavy chain antibodies (Abcam, Cat. No. ab97240, ab97245, ab97250, ab97260) with 1:10,000 dilution was used to detect specific IgG subclass. P. berghei infection P.

(6) A candidate subunit vaccine induces protective immunity against Mycobacterium avium subspecies paratuberculosis in miceNPJ vaccinesMay 20, 2023Shao M, Cui N, Tang Y, Chen F, Cui Y, Dang G, Liu S.peroxidase (HRP) conjugated rabbit or goat anti-mouse IgG (ab6728)/IgM (ab97230)/IgG1 (ab97240)/IgG2a (ab97245) (1:10,000 dilution in PBST, Abcam, Cambridge, MA, USA) were used as the secondary antibodies and incubated

(7) Tubeimuside I improves the efficacy of a therapeutic Fusobacterium nucleatum dendritic cell-based vaccine against colorectal cancerFrontiers in ImmunologyMay 3, 2023Yanan Tong, Guoxiu Lu, Zhiguo Wang, Shanhu Hao, Guoxu Zhang, Hongwu SunPEPROTECH. Goat anti-mouse IgG1 heavy chain (HRP) (ab97240), goat anti-mouse IgG2a heavy chain (HRP) (ab97245), and goat anti-mouse IgG2b heavy chain (HRP) (ab98703) antibodies were purchased from Abcam. A mouse

(8) Adenovirus Vaccine Containing Truncated SARS-CoV-2 Spike Protein S1 Subunit Leads to a Specific Immune Response in MiceVaccinesFebruary 13, 2023Chen K, Shi D, Li C, Fang Z, Guo Y, Jiang W, Li J, Li H, Yao H.(Mouse Anti-Adenovirus Hexon, AbD Serotec 04000079, 1:2000) and secondary (Goat pAb to MS IgG2a(HRP), Abcam ab97245, 1:1000) antibodies were added and incubated for 1 h, during which PBS was used for washing. After incubation,

(9) A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigensNature communicationsJanuary 28, 2023Weyant KB, Oloyede A, Pal S, Liao J, Jesus MR, Jaroentomeechai T, Moeller TD, Hoang-Phou S, Gilmore SF, Singh R, et al.ab6789); anti-mouse IgG1 (1:10,000; Abcam Cat # ab97240), or anti-mouse IgG2a (1:10,000; Abcam Cat # ab97245). Following 3 final washes with PBST, 1-Step Ultra TMB-ELISA (Thermo-Fisher) was added and the plate

(10) Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challengeBiomaterialsJanuary 1, 2023Sun S, Li E, Zhao G, Tang J, Zuo Q, Cai L, Xu C, Sui C, Ou Y, Liu C, et al.peroxidase (HRP) conjugated goat anti-mouse IgG (Ab231712), IgA (Ab97235), IgG1 (ab97240), or IgG2a (Ab97245) (1:10,000, Abcam, UK) and incubated for 40 min at 37 °C. Plates were then washed three-times and added

(11) A critical period of translational control during brain development at codon resolutionNature structural & molecular biologyDecember 1, 2022Harnett D, Ambrozkiewicz MC, Zinnall U, Rusanova A, Borisova E, Drescher AN, Couce-Iglesias M, Villamil G, Dannenberg R, Imami K, et al.HRP secondary antibodies were used at a dilution of 1:2,500: HRP anti-mouse heavy chain (goat, Abcam, ab97245; RRID:AB_10680049) and HRP anti-rabbit heavy chain (goat, Cell Signaling Technology, 7074S, RRID:AB_2099233). Histological

(12) Envelope virus-mimetic nanovaccines by hybridizing bioengineered cell membranes with bacterial vesiclesiScienceJune 17, 2022Mengmeng Zhang, Lu Wang, Jinyao Liu, Yan Panganti-mouse IgG1,Abcam,Cat# ab97240; RRID:AB_10695944 HRP-conjugated goat anti-mouse IgG2a,Abcam,Cat# ab97245; RRID:AB_10680049 anti-mouse CD16/CD32 (clone 93),Biolegend,Cat# 101302; RRID:AB_312801 PE/Cy7 anti-mouse

(13) A taRNA vaccine candidate induces a specific immune response that protects mice against Chikungunya virus infectionsMolecular Therapy - Nucleic AcidsJune 14, 2022Christin Schmidt, Erik Haefner, Julia Gerbeth, Tim Beissert, Ugur Sahin, Mario Perkovic, Barbara S. Schnierlegoat-anti-mouse IgG1 (1:5,000; Abcam, Cambridge, UK; no. ab97240), goat-anti-mouse IgG2a (1:5,000; Abcam; no. ab97245), goat-anti-mouse IgG2b (1:5,000; Abcam; no. ab97250), or goat-anti-mouse IgG3 (1:5,000; Abcam; no.

(14) Redox-sensitive E2 Rad6 controls cellular response to oxidative stress via K63-linked ubiquitination of ribosomesCell reportsMay 24, 2022Simões V, Cizubu BK, Harley L, Zhou Y, Pajak J, Snyder NA, Bouvette J, Borgnia MJ, Arya G, Bartesaghi A, et al.(1:6,000-12,000; Cytiva, cat. no. NA934), and anti-mouse IgG2a (1:10,000-1:12,000; Abcam, cat. no. ab97245) secondary antibodies conjugated with HRP and ECL Prime detection reagents were acquired from Cytiva.

(15) Circular RNA vaccines against SARS-CoV-2 and emerging variantsCellMay 12, 2022Liang Qu et al.IgG,Immunoway,Cat#RS030204 Goat Anti-Mouse IgG1 (HRP),Abcam,Cat#ab97240; RRID:AB_10695944 Goat Anti-Mouse IgG2a (HRP),Abcam,Cat#ab97245; RRID:AB_10680049 Goat Anti-Mouse IgG2c (HRP),Abcam,Cat#ab97255; RRID:AB_10680258 "Anti-Mouse CD3

(16) Vesicular Stomatitis Virus-Based Epstein-Barr Virus Vaccines Elicit Strong Protective Immune ResponsesJournal of VirologyMay 11, 2022Xiang-Wei Kong et al.IgE, and IgM were detected by horseradish peroxidase (HRP)-conjugated antibodies (ab6789, ab97240, ab97245, ab97250, ab97255, ab97260, ab97235, ab99574, and ab97230; Abcam) diluted at a ratio of 1:10,000 with

(17) Identification of B‐cell dominant epitopes in the recombinant protein P29 from Echinococcus granulosusImmunity, Inflammation and DiseaseMay 1, 2022Yongxue Lv, Shasha Li, Tingrui Zhang, Yazhou Zhu, Jia Tao, Jihui Yang, Liangliang Chang, Changyou Wu, Wei Zhaoanti‐mouse IgM, IgG, IgA, IgG1, IgG2a, IgG2b, IgG2c, and IgG3 (Cat nos.: ab97230, ab97023, ab97235, ab97240, ab97245, ab97250, ab97255, and ab97260, respectively; Abcam), and IgE (Cat no.: PA1‐84764; Invitrogen) were

(18) Induction of neutralizing antibodies against SARS-CoV-2 variants by a multivalent mRNA-lipid nanoparticle vaccine encoding SARS-CoV-2/SARS-CoV Spike protein receptor-binding domainsbioRxiv : the preprint server for biologyApril 28, 2022Zhang Q, Tiwari SK, Wang S, Wang L, Li W, Zhang L, Rawlings SA, Cheng Y, Jokerst JV, Rana TM.ab6789, 1:10,000), goat anti-mouse IgG1 (Abcam, ab97240, 1:10,000), or goat anti-mouse IgG2a (Abcam, ab97245, 1:10,000) for 1 h. The plates were washed with PBST, color was developed by addition of 3′,5,5′-tetramethylbenzidine

(19) Novel Synthetic Lipopeptides as Potential Mucosal Adjuvants Enhanced SARS-CoV-2 rRBD-Induced Immune ResponseFrontiers in ImmunologyMarch 9, 2022Ling Mao et al.(ab97235), Goat Anti-Mouse IgG1 heavychain (HRP) (ab97240), Goat Anti-Mouse IgG2a heavy chain (HRP) (ab97245), TMB ELISA Substrate (ab171523) and 450nm stop solution for TMB substrate (ab171529) were purchased

(20) CCN2 participates in overload-induced skeletal muscle hypertrophyMatrix BiologyFebruary 1, 2022Jennifer M. Petrosino, Jacob Z. Longenecker, Colin D. Angell, Scott A. Hinger, Colton R. Martens, Federica Accornerowith the exception of Puromycin blots, which were incubated with anti-mouse HRP conjugated (Abcam, ab97245, 1:10,000 in 5% nonfat milk). All blots were imaged using the ChemiDoc system (Bio-Rad) as previously

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10/13/2015Antibody Reacts With Mouse IgG2a Strongly Ming LuIn my test result, Ab97245 reacts with mouse IgG2a heavy chain strongly. Ab97245 reacts with mouse IgG2a light chain slightly, which is the reason I did not give it a 5-star review. Ab97245 does not ... Read Review

Goat Anti-Mouse IgG2a heavy chain (HRP) from Abcam

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Goat Anti-Mouse IgG2a heavy chain (HRP) from Abcam

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