Anti-ME1 antibody ab97445 from Abcam

Product Specs
ItemAnti-ME1 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab97445
Quantity50 UL
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot
Antibody TypePrimary
Conjugate/TagUnconjugated
IsotypeIgG
ClonalityPolyclonal
HostRabbit
Concentration1 mg/ml
Gene NameMe1
ReactivityHuman
TargetME1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Integrated landscape of cardiac metabolism in end-stage human nonischemic dilated cardiomyopathyNature Cardiovascular ResearchFebruary 13, 2023Emily Flam, Cholsoon Jang, Danielle Murashige, Yifan Yang, Michael P. Morley, Sunhee Jung, Daniel S. Kantner, Hannah Pepper, Kenneth C. Bedi, Jeff Brandimarto, et al.no. ab 110336), AKR1B1 (Thermo Fisher Scientific, catalog no. 15439–1-AP), ME1 (Abcam, catalog no. ab97445), ME3 (Abcam, catalog no. ab172972), HK1 (Cell Signaling Technology, catalog no. 2024S), HK2 (Cell Signaling Technology

(2) Malic Enzyme 1 Absence in Synovial Sarcoma Shifts Antioxidant System Dependence and Increases Sensitivity to Ferroptosis Induction with ACXT-3102Clinical Cancer ResearchAugust 15, 2022Caitlyn B. Brashears, Bethany C. Prudner, Richa Rathore, Katharine E. Caldwell, Carina A. Dehner, Jane L. Buchanan, Sara E.S. Lange, Neal Poulin, Jennifer K. Sehn, Jason Roszik, et al.Slides were heated in antigen unmasking solution (Vector). Primary antibody (1:100 malic enzyme 1, ab97445, Abcam) was applied to each slide and incubated overnight at 4°C. Biotinylated secondary antibody (1:100

(3) Lactate and glutamine support NADPH generation in cancer cells under glucose deprived conditionsRedox BiologyOctober 1, 2021Minfeng Ying, Duo You, Xiaobing Zhu, Limeng Cai, Siying Zeng, Xun Huused here include: anti-IDH1 (1:1000, Abcam, cat. no. ab172964), anti-ME1 (1:2000, Abcam, cat. no. ab97445), anti-MDH1 (1:10000, Abcam, cat. no. ab180152), anti-Visfatin (1:1000, Abcam, cat. no. ab236874), anti-LDHA

(4) Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesisCell metabolismMay 4, 2021Wang YP, Sharda A, Xu SN, van Gastel N, Man CH, Choi U, Leong WZ, Li X, Scadden DT.anti-Flag (clone M2),Sigma-Aldrich,Cat#:F1804; RRID: AB_262044 Rabbit polyclonal anti-ME1,Abcam,Cat#:ab97445; RRID: AB_10679994 Rabbit monoclonal anti-ME2 (clone EP7217),Abcam,Cat#: ab139686 Rabbit monoclonal

(5) Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasisRedox biologySeptember 1, 2020Shao C, Lu W, Du Y, Yan W, Bao Q, Tian Y, Wang G, Ye H, Hao H.antibodies used in this study include antibodies against ME1 (1:1000, 5% non-fat milk, Abcam, cat. no. ab97445), G6PD (1:1000, 5% non-fat milk, Abcam, cat no. ab993), β-actin (1:1000, 5% BSA, Cell Signaling Technology,

(6) CMap analysis identifies Atractyloside as a potential drug candidate for type 2 diabetes based on integration of metabolomics and transcriptomicsJournal of Cellular and Molecular MedicineJuly 1, 2020Hailong Li, Xiaodong Shi, Hua Jiang, Junren Kang, Miao Yu, Qifei Li, Kang Yu, Zhengju Chen, Hui Pan, Wei ChenPVDF membranes were incubated with the primary antibody at 4°C overnight, including anti‐ME1 (1:1000, ab97445, Abcam), anti‐ME2 (1:2000, ab139686, Abcam), and anti‐MDH1 (1:1000, ab181091, Abcam), with β‐actin (1:200,

(7) A small molecule G6PD inhibitor reveals immune dependence on pentose phosphate pathwayNature chemical biologyJuly 1, 2020Ghergurovich JM, García-Cañaveras JC, Wang J, Schmidt E, Zhang Z, TeSlaa T, Patel H, Chen L, Britt EC, Piqueras-Nebot M, et al.Anti-β-actin (5125) was obtained from Cell Signaling Technologies (Danvers, MA). Anti-G6PD (ab993); ME1 (ab97445) and IDH1 (EPR12296) were obtained from Abcam Inc. (Cambridge, MA). CoxIV antibody was obtained from

(8) Malic Enzyme 1 Indicates Worse Prognosis in Breast Cancer and Promotes Metastasis by Manipulating Reactive Oxygen SpeciesOncoTargets and therapyJanuary 1, 2020Liu C, Cao J, Lin S, Zhao Y, Zhu M, Tao Z, Hu X.Buffer (Beyotime, P0260, China) for 15 min, the sections were incubated at 4°C with ME1 antibody (Abcam, ab97445, 1:1000, USA) overnight. The IHC Kit (GTVision, GK500705, China) including second antibody and DAB substrate

(9) Malic enzyme 1 (ME1) is a potential oncogene in gastric cancer cells and is associated with poor survival of gastric cancer patientsOncoTargets and TherapyJuly 11, 2019Yanyan Shi, Siliang Zhou, Pan Wang, Yanlei Guo, Bingteng Xie, Shigang Ding(forward primer) and 5ʹ- CTTGTTCAGGAGACGAAATGC-3ʹ (reverse primer). Primary antibodies for ME1 (1:500, ab97445, Abcam, Cambridge, MA, USA) and β-actin (1:1000, ab8227, Abcam, Cambridge, MA, USA) were used for Western blot

(10) Metabolic reprogramming and Notch activity distinguish between non-small cell lung cancer subtypesBritish journal of cancerJuly 1, 2019Sellers K, Allen TD, Bousamra M 2nd, Tan J, Méndez-Lucas A, Lin W, Bah N, Chernyavskaya Y, MacRae JI, Higashi RM, et al.ab135532, Abcam) PSAT (1:500, 20180-1-AP, Protein Tech) PHGDH (1:1000, sc292792, Santa Cruz) ME1 (1:1000, ab97445, Abcam) ME2 (1:500, ab139686, Abcam) GOT1 (1:250, ap2947a, Abgent) GOT2 (1:500, ab93928, Abcam) IDH1

(11) NADPH production by the oxidative pentose-phosphate pathway supports folate metabolismNature metabolismMarch 1, 2019Chen L, Zhang Z, Hoshino A, Zheng HD, Morley M, Arany Z, Rabinowitz JD.Flag-tag (2368) were obtained from cell Signaling Technologies (Danvers, MA). Anti-G6PD (ab993), ME1 (ab97445), IDH1 (EPR12296) and PGD (EPR6565) were obtained from Abcam Inc. (Cambridge, MA). Anti-DHFR (377091)

(12) HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinomaJournal of cellular and molecular medicineNovember 1, 2017Li W, Chen C, Zhao X, Ye H, Zhao Y, Fu Z, Pan W, Zheng S, Wei L, Nong T, et al.anti‐human GLUD1 antibody (1:1000, #ab168352, Abcam, Cambridge, MA), rabbit anti‐human ME1 antibody(1:1000, #ab97445, Abcam, Cambrigde, MA), mouse anti‐human MDH1 antibody(1:500, #ab76616, Cambrigde, MA) rabbit anti‐human

(13) Cancer-Associated IDH1 Promotes Growth and Resistance to Targeted Therapies in the Absence of MutationCell ReportsMay 27, 2017Andrea E. Calvert et al.CS200603), anti-H3K36me3 (Abcam, ab9050), anti-Histone H3 (Cell Signaling, 4499), anti-ME1 (Abcam, ab97445), anti-FoxO6 (Thermo Scientific PA5–35117), anti-phospho-FoxO6 (Abcam, ab154832), anti-phospho-EGFR

(14) Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usageNature chemical biologyMay 1, 2016Liu L, Shah S, Fan J, Park JO, Wellen KE, Rabinowitz JD.9b,c. The antibodies to the following proteins were purchased from the indicated sources: ME1 (Abcam, ab97445, 1:1,000 dilution), ME2 (Abcam, ab139686, 1:1,000 dilution), ME3 (Abcam, ab172972, 1:2,000 dilution),

(15) The HMGB1 protein induces a metabolic type of tumour cell death by blocking aerobic respirationNature communicationsMarch 7, 2016Gdynia G, Sauer SW, Kopitz J, Fuchs D, Duglova K, Ruppert T, Miller M, Pahl J, Cerwenka A, Enders M, et al.

(16) Activated mTORC1 promotes long-term cone survival in retinitis pigmentosa miceJournal of Clinical InvestigationApril 1, 2015Aditya Venkatesh, Shan Ma, Yun Z. Le, Michael N. Hall, Markus A. Rüegg, Claudio Punzo(Ser422) (1:300; catalog ab55281); rabbit α-G6PD (1:300; catalog ab993); rabbit α-ME1 (1:300; catalog ab97445); rabbit α-Ki67 (1:300; catalog ab15580) (all from Abcam); rabbit α–HIF-1α (1:300; catalog ab1536; R&D

(17) Metabolic efficiency promotes protection from pressure overload in hearts expressing slow skeletal troponin ICirculation. Heart failureJanuary 1, 2015Carley AN, Taglieri DM, Bi J, Solaro RJ, Lewandowski ED.chain, M8424-0.2ML Sigma Aldrich; calsequestrin, PA1-913 Pierce Thermo Scientific; malic enzyme 1, ab97445 Abcam; phospho-AMP kinase α, 2535S Cell Signaling; pyruvate dehydrogenase, ab110330 Abcam; phosphorylated

(18) Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in micePloS oneJanuary 1, 2014Al-Dwairi A, Brown AR, Pabona JM, Van TH, Hamdan H, Mercado CP, Quick CM, Wight PA, Simmen RC, Simmen FA.nitrocellulose membrane. Membranes were incubated overnight with rabbit polyclonal antibody raised against ME1 (ab97445, 1∶1000 dilution; Abcam, Cambridge, MA), rabbit polyclonal antibody raised against FASN (ab22759, 1∶1000

(19) Metabolic intervention on lipid synthesis converging pathways abrogates prostate cancer growthOncogeneOctober 17, 2013Fritz V, Benfodda Z, Henriquet C, Hure S, Cristol JP, Michel F, Carbonneau MA, Casas F, Fajas L.

(20) Dietary soy protein induces hepatic lipogenic enzyme gene expression while suppressing hepatosteatosis in obese female Zucker rats bearing DMBA-initiated mammary tumorsGenes & nutritionOctober 1, 2012Reza Hakkak, Ahmed Al-Dwairi, George J Fuchs, Soheila Korourian, Frank A Simmen

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Anti-ME1 antibody from Abcam

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Anti-ME1 antibody from Abcam

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