Anti-SNRP70/U1-70K antibody ab83306 from Abcam

Product Specs
ItemAnti-SNRP70/U1-70K antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab83306
Quantity100 UG
IsotypeIgG
ClonalityPolyclonal
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Antibody TypePrimary
Conjugate/TagUnconjugated
HostRabbit
Concentration0.4 mg/ml
Gene NameSNRNP70
ReactivityHuman, Mouse, Rat
TargetSNRP70

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Mitotic bookmarking by SWI/SNF subunitsNatureJune 1, 2023Zhu Z, Chen X, Guo A, Manzano T, Walsh PJ, Wills KM, Halliburton R, Radko-Juettner S, Carter RD, Partridge JF, et al.PA5–21079, 1: 1000); BRD9 (Active Motif, 61537, 1: 1000); SMARCA4 (CST, 49360, 1: 1000); SNRP70 (Abcam, ab83306, 1: 1000); BRD7 (CST, 14910, 1: 1000); SMARCC1 (CST, 11956, 1: 1000)) overnight at 4°C. Membranes were

(2) HOTAIR regulates SIRT3-mediated cardiomyocyte survival after myocardial ischemia/reperfusion by interacting with FUSBMC Cardiovascular DisordersMarch 30, 2023Jixuan Liu, Mingzhuang Sun, Jinda Wang, Zhijun Sun, Gang Wangconjugated to antibodies (5 μL) against anti-FUS antibody (ab234880, Abcam), SNRNP70 (positive control; ab83306, Abcam) or immunoglobulin (Ig) G (negative control; Abcam) for 24 h at 4 °C. The HOTAIR in immunoprecipitation

(3) LSM1-mediated Major Satellite RNA decay is required for nonequilibrium histone H3.3 incorporation into parental pronucleiNature communicationsFebruary 21, 2023Zhu J, Chen K, Sun YH, Ye W, Liu J, Zhang D, Su N, Wu L, Kou X, Zhao Y, et al.Scientific, MA5-24667, 1:1000), anti-TUBULIN (Proteintech, 66031-1-Ig, 1:2000), anti-SNRP70 (Abcam, ab83306, 1:1000) and anti-GAPDH (Proteintech, 10494-1-AP, 1:1000). The membranes were washed in TBST, incubated

(4) Analysis of estrogen-regulated enhancer RNAs identifies a functional motif required for enhancer assembly and gene expressionCell ReportsJune 14, 2022Tim Y. Hou, W. Lee Krausas follows: rabbit polyclonal anti-ß-tubulin (Abcam, ab6046), rabbit polyclonal anti-SNRNP70 (Abcam, ab83306), mouse monoclonal anti-Flag (Sigma-Aldrich, F3165), rabbit polyclonal anti-Cas9 (Diagenode, C15310258–100),

(5) ZFC3H1 and U1-70K promote the nuclear retention of mRNAs with 5′ splice site motifs within nuclear specklesRNAJune 1, 2022Eliza S. Lee, Harrison W. Smith, Eric J. Wolf, Aysegul Guvenek, Yifan E. Wang, Andrew Emili, Bin Tian, Alexander F. PalazzoSKIV2L2) (Bethyl Laboratories, A300-614A), PABPN1 (Bethyl Laboratories, A303-523A), U1-70K (Abcam, ab83306), Aly (Zhou et al. 2000), and TRAPα (Görlich et al. 1990) or mouse monoclonals against U1-70K (Sigma-Aldrich,

(6) Physiological tissue-specific and age-related reduction of mouse TDP-43 levels is regulated by epigenetic modificationsDisease models & mechanismsApril 1, 2022Pacetti M, De Conti L, Marasco LE, Romano M, Rashid MM, Nubiè M, Baralle FE, Baralle M.anti-hnRNPQ (Sigma-Aldrich, HPA041275, 1:1000); anti-hnRNPR (Abcam, ab30930, 1:1000); anti-snRNP70 (Abcam, ab83306, 1:1000); anti-1H4 (SR75) (Merck, MABE50, 1:1000); anti-GAPDH (Abcam, ab9485, 1:2500), anti-DNMT1 (Abcam,

(7) The METTL5-TRMT112 N(6)-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylationThe Journal of biological chemistryMarch 1, 2022Sepich-Poore C, Zheng Z, Schmitt E, Wen K, Zhang ZS, Cui XL, Dai Q, Zhu AC, Zhang L, Sanchez Castillo A, et al.dilution anti-GAPDH-HRP,GenScript/A00192-100,"Western blot/goat/1:10,000 dilution" anti-SNRP70,Abcam/ab83306,Western blot/rabbit/1:1000 dilution anti-histone H3,Cell Signaling Technology/D1H2,Western blot/rabbit/1:2000

(8) SETMAR Shorter Isoform: A New Prognostic Factor in GlioblastomaFrontiers in OncologyJanuary 3, 2022Oriane Lié, Thierry Virolle, Mathieu Gabut, Claude Pasquier, Ilyess Zemmoura, Corinne Augé-Gouillou(Ab129455, Abcam, 1/2500), PTN (Ab14025, Abcam, 1/1000), OLIG2 (Ab9610, Milipore, 1/1000) snRNP70 (Ab83306, Abcam, 1/1000), actin-HRP (A3854, Sigma-Aldrich, 1/100 000), α-peptide (custom designed by Covalab;

(9) Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancersCellAugust 19, 2021Challa S, Khulpateea BR, Nandu T, Camacho CV, Ryu KW, Chen H, Peng Y, Lea JS, Kraus WL.GTX123450), mouse monoclonal against NMNAT-2 (Abcam, ab56980), ß-tubulin (Abcam, ab6046), SNRP70 (Abcam, ab83306), RPS6 (Cell Signaling Technologies, 2317S), RPL10 (Biorad, VPA00362), RPS5 (Santa Cruz, sc-390935),

(10) Tau aggregates are RNA-protein assemblies that mislocalize multiple nuclear speckle componentsNeuronMay 19, 2021Lester E, Ooi FK, Bakkar N, Ayers J, Woerman AL, Wheeler J, Bowser R, Carlson GA, Prusiner SB, Parker R.RRID:AB_1140733" Rabbit anti-SRSF2,Abcam,"Cat #: ab204916," Rabbit anti-SNRNP70 (U1-70K),Abcam,"Cat #: ab83306, RRID:AB_10673827" Rabbit anti-PABP,Abcam,"Cat #: ab21060, RRID:AB_777008" Rabbit anti-TIA1,Abcam,"Cat

(11) Stress-induced nuclear condensation of NELF drives transcriptional downregulationMolecular cellMarch 4, 2021Rawat P, Boehning M, Hummel B, Aprile-Garcia F, Pandit AS, Eisenhardt N, Khavaran A, Niskanen E, Vos SM, Palvimo JJ, et al.polyclonal, sc-32912 (H-140), Santa Cruz Biotechnology) at 1:1,000, anti-SNRNP70 (rabbit polyclonal, ab83306, Abcam) at 1:1,000, anti-ZNF451 (rabbit polyclonal, A305-177A, Bethyl Laboratories inc), anti-RPB3 (rabbit

(12) Functional Interplay between Histone H2B ADP-Ribosylation and Phosphorylation Controls AdipogenesisMolecular cellSeptember 17, 2020Huang D, Camacho CV, Setlem R, Ryu KW, Parameswaran B, Gupta RK, Kraus WL.Phospho-p70 S6 kinase Thr389 (Cell Signaling, 9205), p70 S6 kinase (Cell Signaling, 9202), SNRNP70 (Abcam, ab83306), β-actin (Cell Signaling, 3700), rabbit IgG (ThermoFisher, 10500C), goat anti-rabbit HRP-conjugated

(13) ADP-Ribosylation Levels and Patterns Correlate with Gene Expression and Clinical Outcomes in Ovarian CancersMolecular Cancer TherapeuticsJanuary 1, 2020Lesley B. Conrad, Ken Y. Lin, Tulip Nandu, Bryan A. Gibson, Jayanthi S. Lea, W. Lee Kraus(generated in the Kraus lab) (31), used at 1:1000 dilution. Primary antibodies against SNRP70 (Abcam ab83306; 1:4000 dilution) and TFIID (SI-1) (Santa Cruz sc-273; 1:500 dilution) were used for detection of loading

(14) MeCP2 Represses the Rate of Transcriptional Initiation of Highly Methylated Long GenesMolecular CellJanuary 1, 2020Lisa D. Boxer et al.imaged on a Li-Cor Odyssey. Primary antibodies used were: Gapdh (Sigma G9545, 1:5000), Snrp70 (Abcam ab83306, 1:1000 from 0.9 μg/μL variable stock), Histone H3 (Abcam ab1791, 1:5000), MeCP2 (Chen et al., 2003),

(15) Long non‑coding RNA LINC00473/miR‑195‑5p promotes glioma progression via YAP1‑TEAD1‑Hippo signalingInternational Journal of OncologyDecember 18, 2019Xuan Wang, Xu Dong Li, Zhenyuan Fu, Yan Zhou, Xing Huang, Xiaobing JiangAbcam) was used as the negative control, and anti-U1 small nucleoprotein 70 kDa (SNRNP70; 1:50; cat. no. ab83306; Abcam) was used as positive control. RNA was subsequently isolated for reverse transcription (RT)-qPCR,

(16) Regulation of Co-transcriptional Pre-mRNA Splicing by m⁶A through the Low-Complexity Protein hnRNPGMolecular cellOctober 3, 2019Zhou KI, Shi H, Lyu R, Wylder AC, Matuszek Ż, Pan JN, He C, Parisien M, Pan T.Proteintech), 1/1000 v/v anti-GST antibody (2624, Cell Signaling Technology), 1/3000 v/v anti-SNRP70 antibody (ab83306, Abcam), 1/5000 v/v anti-H3K4me3 (ab8580, Abcam), or 0.05 μg/mL anti-GAPDH antibody (A00192–40, Genscript),

(17) E47 modulates hepatic glucocorticoid actionNature communicationsJanuary 18, 2019Hemmer MC, Wierer M, Schachtrup K, Downes M, Hübner N, Evans RM, Uhlenhaut NH.and E47−/− livers was performed using rabbit αGR (#12041, Cell Signaling, 1:2000) and rabbit αSnrp70 (ab83306, Abcam, 1:5000) antibodies. Histology For Oil Red O stainings on PFA-fxed liver tissue, 6μm cryosections

(18) lncRNA Epigenetic Landscape Analysis Identifies EPIC1 as an Oncogenic lncRNA that Interacts with MYC and Promotes Cell-Cycle Progression in CancerCancer cellApril 9, 2018Wang Z, Yang B, Zhang M, Guo W, Wu Z, Wang Y, Jia L, Li S, Cancer Genome Atlas Research Network., Xie W, et al.approaches. Antibodies The following antibodies were used for immunoblotting: rabbit anti-SNRP70 (Abcam, #ab83306), rabbit anti-GAPDH (Santa Cruz, #sc-25778), rabbit anti-MYC (Cell Signaling, #13987), rabbit anti-p21

(19) Therapeutic activity of modified U1 core spliceosomal particlesNature CommunicationsSeptember 1, 2016Malgorzata Ewa Rogalska, Mojca Tajnik, Danilo Licastro, Erica Bussani, Luca Camparini, Chiara Mattioli, Franco Paganiefficiency by western blot, using monoclonal antibodies against U1C (4H12, ab122901 Abcam, 1:1,000), U1-70K (ab83306, Abcam. 1:1,000) and U1A (ab55751, Abcam, 1:5,000). Uncropped blots are shown in Supplementary Fig.

(20) The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved SRA1-like RegionCell ReportsMay 1, 2016Karyn Schmidt, Cailin E. Joyce, Frank Buquicchio, Adam Brown, Justin Ritz, Robert J. Distel, Charles H. Yoon, Carl D. Novina(N-20), Brn3a (14A6), Hsp90 (4F10), and rabbit IgG control; Cell Signaling GAPDH (14C10); Abcam SNRNP70 (ab83306); and BD Pharmingen™ mouse IgG control. Santa Cruz AR (H-280) was used for ChIP PCR. Sequences for all

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Anti-SNRP70/U1-70K antibody from Abcam

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Anti-SNRP70/U1-70K antibody from Abcam

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