Anti-Sp7 / Osterix antibody ab94744 from Abcam

Product Specs
ItemAnti-Sp7 / Osterix antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab94744
Quantity100 UL
Conjugate/TagUnconjugated
HostRabbit
Antibody TypePrimary
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
ClonalityPolyclonal
IsotypeIgG
Concentration1 mg/ml
Gene NameSP7
ReactivityHuman
TargetSp7 / Osterix

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Website: http://www.abcam.com

(1) Runx2 overexpression promotes bone repair of osteonecrosis of the femoral head (ONFH)Molecular Biology ReportsJune 1, 2023Hai-Jia Xu, Xiang-Zhong Liu, Lu Yang, Yu Ning, Liang-Liang Xu, Da-Ming Sun, Wen Liao, Yi Yang, Zhang-Hua LiWestern blot analysis was used to detected the protein level of BMP-2 (ab6285), Runx2 (ab114133), Osterix (ab94744) (all from Abcam, USA) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, ab9485). Total protein was

(2) Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β-catenin signallingJournal of cellular and molecular medicineApril 1, 2023Wang Y, Gan Y, Dong Y, Zhou J, Zhu E, Yuan H, Li X, Wang B.anti‐low‐density lipoprotein receptor‐related protein 6 (LRP6) (ab134146), anti‐ALP (ab108337), and anti‐osterix (ab94744); antibodies by Cell Signalling Technology: anti‐PPARγ (#2443), anti‐C/EBPα (#8178); anti‐Runx2 (#12556),

(3) Ferutinin induces osteoblast differentiation of DPSCs via induction of KLF2 and autophagy/mitophagyCell Death & DiseaseMay 12, 2022Jyotirindra Maity, Derek Barthels, Jaganmay Sarkar, Prateeksha Prateeksha, Moonmoon Deb, Daniela Rolph, Hiranmoy Das(20–188) and osteocalcin antibody (AB10911) were purchased from Millipore. Parkin (ab77924), Osterix (ab94744), osteopontin (ab8448), H3K4me3 (ab1012), H3K27Ac (ab4729), KLF2 (ab203591), and goat IgG (ab37373)

(4) Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1Nature communicationsMay 5, 2022Xu C, Zhang Z, Liu N, Li L, Zhong H, Wang R, Shi Q, Zhang Z, Wei L, Hu B, et al.antibodies were used (all at a 1:1,000 dilution): rabbit anti-RUNX2 (ab23981, Abcam), rabbit anti-OSX (ab94744, Abcam), rabbit anti-OCN (23418-1-AP, ProteinTech, Wuhan, China), rabbit anti-ALP (ab83259, Abcam),

(5) Implantable Electrical Stimulation at Dorsal Root Ganglions Accelerates Osteoporotic Fracture Healing via Calcitonin Gene-Related PeptideAdvanced science (Weinheim, Baden-Wurttemberg, Germany)January 1, 2022Mi J, Xu JK, Yao Z, Yao H, Li Y, He X, Dai BY, Zou L, Tong WX, Zhang XT, et al.microscope (Leica Q500MC, Leica, Germany). CGRP (1:200, ab5694, Abcam, Cambridge, MA, USA), SP7 (1:200; ab94744; Abcam,Cambridge, MA, USA), OCN (1:400; PA5‐78871; Thermo Fisher Scientific, Waltham, MA), and corresponding

(6) Quercetin Attenuates Osteoporosis in Orchiectomy Mice by Regulating Glucose and Lipid Metabolism via the GPRC6A/AMPK/mTOR Signaling PathwayFrontiers in endocrinologyJanuary 1, 2022Sun J, Pan Y, Li X, Wang L, Liu M, Tu P, Wu C, Xiao J, Han Q, Da W, et al.anti-p-AMPK (1:2000; CST, 50081), anti-RUXN2 (1:4000; Proteintech, 20700-1-AP), anti-Osterix (1:4000; Abcam, ab94744), and anti-GAPDH (1:5000; Proteintech, 10494-1-AP) overnight at 4°C and then incubated with the secondary

(7) Baicalein inhibits inflammatory response and promotes osteogenic activity in periodontal ligament cells challenged with lipopolysaccharidesBMC Complementary Medicine and TherapiesDecember 1, 2021Manman Ren, Ya Zhao, Zhiqi He, Jian Lin, Chuchu Xu, Fen Liu, Rongdang Hu, Hui Deng, Yi Wang4970, CST, USA), Collagen-I (COL-I, 1:1000, No. sc-8785, Santa Cruz, USA), Osterix (OSX, 1:2000, No. ab94744, Abcam, USA), ERK (1:1000, No. 4695, CST, USA), phospho-ERK (p-ERK, 1:1000, No. 4370, CST, USA), p38

(8) Conditioned medium derived from 3D tooth germs: A novel cocktail for stem cell priming and early in vivo pulp regenerationCell ProliferationNovember 1, 2021Tengfei Zhou, Mingdeng Rong, Zijie Wang, Hongxing Chu, Chuying Chen, Jiayi Zhang, Zhihui Tian(ab103203, Abcam), DSPP (sc‐73632, Santa Cruz Biotechnology, Dallas, TX), BSP (ab52128, Abcam), OSX (ab94744, Abcam), and VEGF (sc‐57496, Santa Cruz Biotechnology), with GAPDH as an internal control. Membranes

(9) Overexpression of COMP-Angiopoietin-1 in K14-Expressing Cells Impairs Hematopoiesis and Disturbs Erythrocyte MaturationMolecules and CellsApril 30, 2021Hyun-Jaung Sim, Min-Hye Kim, Govinda Bhattarai, Jae-Won Hwang, Han-Sol So, Sher Bahadur Poudel, Eui-Sic Cho, Sung-Ho Kook, Jeong-Chae Leerabbit-anti-runt-related transcription factor 2 (Runx2) (cat.#BS2831; Bioworld Technology, USA), rabbit-anti-osterix (cat.#ab94744; Abcam), or mouse-anti-monoclonal β-actin (cat.#sc-81178, 1:200; Santa Cruz Biotechnology) at 4°C. Membranes

(10) Comparison of Osteogenic Differentiation Potential of Human Dental-Derived Stem Cells Isolated from Dental Pulp, Periodontal Ligament, Dental Follicle, and Alveolar BoneStem Cells InternationalApril 7, 2021Guanlin Qu, Yan Li, Lu Chen, Qin Chen, Duohong Zou, Chi Yang, Qing Zhouprimary antibodies RUNX2 (1 : 2000, ab236639, Abcam), ALP (1 : 2000, ab83259, Abcam), OSX (1 : 2000, ab94744, Abcam), OPN (1 : 2000, ab8448, Abcam), and β-actin (1 : 2000, ab8227, Abcam) overnight at 4°C. After

(11) Wnt7a promotes the osteogenic differentiation of human mesenchymal stem cellsInternational Journal of Molecular MedicineApril 6, 2021Leiluo Yang, Qing Li, Junhong Zhang, Pengcheng Li, Pingjiang An, Chunqing Wang, Pingsheng Hu, Xue Zou, Xiaowei Dou, Lixin ZhuOCN (ab13418; Abcam), OPN (ab214050; Abcam), RUNX2 (12556, Cell Signaling Technology, Inc.) and OSX (ab94744, Abcam) at 1:1,000 overnight at 4°C. The membranes were rinsed three times by TBST and incubated with

(12) Exosomal miR-365a-5p derived from HUC-MSCs regulates osteogenesis in GIONFH through the Hippo signaling pathwayMolecular therapy. Nucleic acidsMarch 5, 2021Kuang MJ, Zhang KH, Qiu J, Wang AB, Che WW, Li XM, Shi DL, Wang DC.(Abcam, 125011), anti-GAPDH (Proteintech, 10494-1-AP), anti-BMP2 (Abcam, ab14933), anti-Sp7 (Abcam, ab94744), anti-SAV1 (Abcam, ab172705), anti-p-LATS (Abcam, ab111344), anti-YAP (Abcam, ab52771), anti-p-YAP

(13) The Toll-like receptor ligand, CpG oligodeoxynucleotides, regulate proliferation and osteogenic differentiation of osteoblastJournal of Orthopaedic Surgery and ResearchDecember 1, 2020Wenwen Yu, Yi Zheng, Hongyan Li, Hongbing Lin, Zhen Chen, Yue Tian, Huishan Chen, Peipei Zhang, Xiaowei Xu, Yuqin Shenprimary antibodies at 4 °C overnight: cyclin D1 (1:500, A2708, ABclonal, Wuhan, China), SP7 (1:1000, ab94744, Abcam, Shanghai, China), OCN (1:650, A5786, ABclonal), Runx2 (1:500, A2851, ABclonal), P27Kip1 (1:500,

(14) KLF2 regulates dental pulp-derived stem cell differentiation through the induction of mitophagy and altering mitochondrial metabolismRedox BiologySeptember 1, 2020Jyotirindra Maity, Moonmoon Deb, Carl Greene, Hiranmoy Daslysis buffer (20–188) and antibody for osteocalcin (AB10911) were purchased from Millipore. Osterix (ab94744), osteopontin (ab8448), H3K4me3 (ab1012), H3K27Ac (ab4729), KLF2 (ab203591), goat IgG (ab37373) were

(15) Neuroblastoma‐secreted exosomes carrying miR‐375 promote osteogenic differentiation of bone‐marrow mesenchymal stromal cellsJournal of Extracellular VesiclesSeptember 1, 2020Marta Colletti et al.antibody [EP1675Y] ab76252 (Abcam); anti-YAP antibody [EP1674Y] ab52771 (Abcam); Anti-Sp7/Osterix antibody ab94744 (abcam); anti-BMP2 antibody ab14933 (abcam); RUNX2 (D1L7 F) Rabbit mAb #12,556 (Cell Signalling Technology);

(16) miR‑27a‑3p negatively regulates osteogenic differentiation of MC3T3‑E1 preosteoblasts by targeting osterixMolecular Medicine ReportsJune 17, 2020Yuexin Xu, Dong Li, Zhu Zhu, Lingyun Li, Yucui Jin, Changyan Ma, Wei Zhangsoftware (version 1.49v; National Institutes of Health). Primary antibodies against Osx (1:4,000; cat. no. ab94744; Abcam) and β-actin (1:1,000; cat. no. sc-47778; Santa Cruz Biotechnology, Inc.) were used. Plasmid

(17) Overexpressing of POU2F2 accelerates fracture healing via regulating HMGA1/Wnt/β-catenin signaling pathwayBioscience, Biotechnology, and BiochemistryMarch 3, 2020Wei Zhang, Hanke Zhao, Kun Chen, Ye Huangantibodies including anti-HMGA1 (ab129153), anti-Col10a1 (ab58632), antiRunx2 (ab76956), anti-Osterix (ab94744), antiOsteocalcin (ab236048) and anti-wnt10b (ab70816) were obtained from Abcam Techonology (USA). Primary

(18) An Improved Methodology to Evaluate Cell and Molecular Signals in the Reparative Callus During Fracture HealingThe journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyMarch 1, 2020Valiya Kambrath A, Williams JN, Sankar U.chondrocyte progenitors SOX9 AB5535, Millipore Sigma 1:50 Hypertrophic chondrocytes COL10 234196, Millipore 1:400 OSX AB94744, Abcam 1:50 AB765P, EMD Millipore 1:100 PA5-42706, Thermo Fisher Scientific 1:100 Osteoblast progenitors Mature

(19) Inflammatory macrophages facilitate mechanical stress-induced osteogenesisAging (Albany NY)February 25, 2020Fan Zhang, Le Huan, Tao Xu, Guozheng Li, Bing Zheng, Hong Zhao, Yongfei Guo, Jiangang Shi, Jingchuan Sun, Aimin Chenanti-Sox9 (sc-166505, Santa Cruz Biotechnology, Dallas, TX, USA) or rabbit polyclonal anti-Osterix (ab94744, Abcam) overnight at 4 °C, followed by anti-goat, anti-mouse or anti-rabbit secondary antibodies (Jackson

(20) Multiple myeloma-derived exosomes inhibit osteoblastic differentiation and improve IL-6 secretion of BMSCs from multiple myelomaJournal of Investigative MedicineJanuary 1, 2020Zhaoyun Liu, Hui Liu, Yanqi Li, Qin Shao, Jin Chen, Jia Song, Rong FuAnti-CD63 (Abcam, TS63), anti-Hsp70 (Abcam, 3A3), anti-CD138 (Abcam, EPR6454), anti-Osterix (Abcam, ab94744), and OCN (Abcam, ab133612) antibodies were used. Western blot was also used to evaluate the content

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Anti-Sp7 / Osterix antibody from Abcam

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Anti-Sp7 / Osterix antibody from Abcam

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