Fig 2: TRIM2 gene is highly expressed in lung adenocarcinoma tissues. a mRNA abundance analysis of TRIM2 gene in GEPIA database. b mRNA abundance analysis of TRIM2 gene in oncomine database. c Western blot analysis of normal tissues and ung adenocarcinoma tissues. N: normal tissues; T: tumor tissues. GAPDH was employed as an internal reference. d qRT-PCR analysis the TRIM2 mRNA abundance in four lung adenocarcinoma tissues and paired normal tissues. e, f Immunohistochemical analysis of normal tissues and lung adenocarcinoma tissues. ***P < 0.001

Fig 3: Regulation of TRIM2 on lung adenocarcinoma cell migration and invasion. a Cell scratch test of TRIM2 knockdown treated A549 cells. b Cell scratch test of TRIM2 overexpression treated H322 cells. c, d Cell migration and invasion analysis of TRIM2 knockdown treated A549 cells and TRIM2 overexpression treated H322 cells, respectively. e Western blot analysis of EMT-related biomarkers expression in TRIM2 knockdown treated A549 cells and TRIM2 overexpression treated H322 cells. f qRT-PCR analysis of EMT-related biomarkers expression in TRIM2 knockdown treated A549 cells and TRIM2 overexpression treated H322 cells. **P < 0.01, ***P < 0.001

Fig 4: TRIM2 promotes proliferation of lung adenocarcinoma cells in vitro. a Western blot analysis of TRIM2 expression in HNBE, H322, H1299, H460, H1703, and A549 cells. b qRT-PCR analysis of TRIM2 expression in HNBE, H322, H1299, H460, H1703, and A549 cells. c TRIM2 protein expression of the TRIM2 knockdown treated A549 cells and TRIM2 overexpression treated H322 cells. d TRIM2 mRNA expression of the TRIM2 knockdown treated A549 cells and TRIM2 overexpression treated H322 cells. e Cell proliferation analysis of TRIM2 knockdown treated A549 cells. f Cell proliferation analysis of TRIM2 overexpression treated H322 cells. g Colony formation analysis of the TRIM2 knockdown treated A549 cells and TRIM2 overexpression treated H322 cells. h, i Western blot analysis of cell proliferation-related biomarkers expression in TRIM2 knockdown treated A549 cells and TRIM2 overexpression treated H322 cells.**P < 0.01, ***P < 0.001

Fig 5: TRIM2 regulates Snail1 expression through ubiquitination. a Correlation analysis of TRIM2 mRNA and Snail1 mRNA in GEPIA database. b Correlation analysis of TRIM2 mRNA and Snail1 mRNA in clinical samples. c Western blot analysis of Snail1 protein expression in TRIM2 knockdown treated A549 cells and TRIM2 overexpression treated H322 cells. d qRT-PCR analysis of Snail1 protein expression in TRIM2 knockdown treated A549 cells and TRIM2 overexpression treated H322 cells. e Correlation analysis of TRIM2 protein expression and Snail1 protein expression in clinical samples. f Western blot analysis of TRIM2 and Snail1 expression in TRIM2 over-expression treated H322 cells with CQ and MG132 treatment on different time points. g CO-IP analysis of TRIM2 and Snail1 interaction in knockdown treated A549 cells. h Forward and reverse CO-IP analysis of TRIM2 and Snail1 in H322 cells and A549 cells. i Immunocolocalization analysis of TRIM2 and Snail1 in H322 cells and A549 cells. j, k Rrmaining Snail1 protein in H322 cells and A549 cells with cyclohexane (CHX) treatment on different time points. l, m Ubiquitination test of TRIM2 and Snail1 in A549 cells and H322 cells, respectively. ***P < 0.001