Fig 1: Confirmation of the loss of synaptic genes in PFC of Alzheimer’s disease humans. (A) Bar graphs showing qPCR data of the selected synaptic genes in PFC (BA10) of postmortem tissues from Alzheimer’s disease patients vs. control (Ctrl: n = 6, Alzheimer’s disease: n = 6). (B) Representative Western blots and quantification of synaptic proteins (STX1A, SNAP25, VAMP2 and PSD95) in the synaptic fraction of PFC from Alzheimer’s disease patients vs. control subjects (Ctrl: n = 5, Alzheimer’s disease: n = 6). (C) Representative immunofluorescence images of synaptic proteins (STX1A, SNAP25, VAMP2), co-stained with the neuronal marker NeuN, in PFC from Alzheimer’s disease patients vs. control subjects. scale bar, 50 µm. (D) Quantification of the fluorescence average intensity and puncta area of synaptic proteins in Alzheimer’s disease and controls (n = 9 slices from 3 humans each group). All data are presented as mean ± SEM. In all figures, *: P < 0.05, **: P < 0.01, ***: P < 0.001, t-test.