Anti DYKDDDDK tag, Monoclonal Antibody 012-22384 from FUJIFILM Wako Chemicals U.S.A. Corp

Product Specs
ItemAnti DYKDDDDK tag, Monoclonal Antibody
CompanyFUJIFILM Wako Chemicals U.S.A. Corp
Price Supplier Page
Catalog Number012-22384
FormatLiquid
Clone1E6
TargetDYKDDDDK tag
ClonalityMonoclonal Antibody
Quantity5 mg (also 1 mg and 200 µg)
Conjugate/TagUnconjugated
ApplicationsWB,IP
IsotypeIgG2b
HostMouse

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FUJIFILM Wako Chemicals U.S.A. Corp

1600 Bellwood Road

Richmond, VA 23237-1326

United States

Phone: 804-271-7677
Fax: 804-271-7791

Email: wkuslabchem@fujifilm.com

Company Profile

Website: www.wakousa.com

(1) CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signalingNucleic acids researchMay 22, 2023Matsumoto M, Modliszewski JL, Shinozaki K, Maezawa R, Perez VM, Ishikawa Y, Suzuki R, McKnight KL, Masaki T, Hirai-Yuki A, et al.(1:500 dilution, #6943) were from Cell Signaling Technology; DYKDDDDK (Clone 1E6, 1:1000 dilution, 018–22381) and GAPDH were from Wako (Clone 5A12; 1:5000 dilution, 016-25523); CSNK2B (1:2000 dilution, A301-984A)

(2) Actin filaments accumulated in the nucleus and remain in the vicinity of condensing chromosomes during prophase in zebrafish early embryoBiology OpenMay 15, 2023Haruka Oda, Yuko Sato, Shigehiro A. Kawashima, Yusuke Fujiwara, Máté Pálfy, Edlyn Wu, Nadine L. Vastenhouw, Motomu Kanai, Hiroshi KimuraFabs [anti-H3K9ac (CMA315; 50 pg), anti-H3S28ph (CMA315; 50 pg); anti-DYKDDDDK tag (FUJIFILM Wako, 012-22384; 50 pg); Sato et al., 2019] and 10 fmol JF646-LANA (see below) in 0.5 nL phosphate-buffered saline (PBS,

(3) The association of UBAP2L and G3BP1 mediated by small nucleolar RNA is essential for stress granule formationCommunications BiologyApril 14, 2023Eri Asano-Inami, Akira Yokoi, Mai Sugiyama, Toshinori Hyodo, Tomonari Hamaguchi, Hiroaki Kajiyama(Santa Cruz, CA, USA); anti-DCP1A antibody (H00055802-M06), Abnova (Taipei, Taiwan); anti-FLAG antibody (014-22383), Wako (Osaka, Japan); anti-GFP (598) and anti-DIG (M227-3) antibodies were obtained from MBL (Nagoya,

(4) CCPG1 recognizes endoplasmic reticulum luminal proteins for selective ER-phagyMolecular biology of the cellApril 1, 2023Ishii S, Chino H, Ode KL, Kurikawa Y, Ueda HR, Matsuura A, Mizushima N, Itakura E.Japan). Mouse monoclonal anti-β-actin (281-98721), anti-GFP (clone mFX75, 012-22541), and anti-FLAG (018-22381) antibodies were purchased from Wako (Osaka, Japan). Mouse monoclonal anti-RPL19 (WH0006143M1), anti-β-actin

(5) Mitochondria regulate intracellular coenzyme Q transport and ferroptotic resistance via STARD7Nature cell biologyFebruary 1, 2023Deshwal S, Onishi M, Tatsuta T, Bartsch T, Cors E, Ried K, Lemke K, Nolte H, Giavalisco P, Langer T.SMAC (MBL, JM-3298-100) dilution 1:1,000, STARD7 (Proteintech 15689-1-AP) dilution 1:2,500, FLAG (WAKO 018-22381) dilution 1:1,000, SDHA (Abcam Ab14715) dilution 1:10,000, He) dilution 1:1,000, CLPP (Sigma HPA010649)

(6) Regulation of A-to-I RNA editing and stop codon recoding to control selenoprotein expression during skeletal myogenesisNature CommunicationsDecember 1, 2022Yuta Noda, Shunpei Okada, Tsutomu SuzukiSanta Cruz Biotechnology), anti-ADAR2 (1:500) (sc-73409, Santa Cruz Biotechnology), anti-DYKDDDDK (FLAG) (1:6000) (014-22383, Wako), anti-GAPDH (1:1000) (AM4300, Invitrogen), anti-GPX1 (1:500) (sc-133160, Santa Cruz Biotechnology),

(7) Imaging translational control by Argonaute with single-molecule resolution in live cellsNature CommunicationsDecember 1, 2022Charlotte A. Cialek, Gabriel Galindo, Tatsuya Morisaki, Ning Zhao, Taiowa A. Montgomery, Timothy J. Stasevichpurified as previously described9. Briefly, Fab were generated from monoclonal α-FLAG antibodies (Wako, 012-22384 Anti DYKDDDDK mouse IgG2b) using the Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit (Thermo Fisher).

(8) m6A RNA methylation regulates the transcription factors JUN and JUNB in TGF-β-induced epithelial-mesenchymal transition of lung cancer cellsThe Journal of biological chemistryNovember 1, 2022Suphakhong K, Terashima M, Wanna-Udom S, Takatsuka R, Ishimura A, Takino T, Suzuki T.protease inhibitors and SUPERase-In. The lysates were treated with anti-FLAG antibody (catalog no.: 018-22381; Wako) or normal mouse IgG bound to Dynabeads M-280 sheep antimouse IgG (Invitrogen). The coprecipitated

(9) Tight junction formation by a claudin mutant lacking the COOH-terminal PDZ domain-binding motifAnnals of the New York Academy of SciencesOctober 1, 2022Fujiwara S, Nguyen TP, Furuse K, Fukazawa Y, Otani T, Furuse M.rabbit polyclonal anti‐claudin‐3 (#34‐1700; Thermo Fisher Scientific), mouse monoclonal anti‐FLAG (#014‐22383; WAKO), rat monoclonal anti‐occludin (clone MOC37), 16 mouse monoclonal anti‐ZO‐1(T8‐754), 17 rat

(10) Inner nuclear membrane protein TMEM201 maintains endothelial cell migration and angiogenesis by interacting with the LINC complexJournal of Molecular Cell BiologyJuly 14, 2022Yutian Zhang, Ya Kong, Haoran Guo, Yun Liu, Yi Zang, Jia Limaterials The antibodies and fluorescent dyes used in this study were as follows: anti-Flag (FUJIFILM Wako, 018-22381), normal mouse IgG (Santa Cruz, sc-2025), and calcein AM (MB5279, Meilunbio). Anti-GAPDH (14C10) rabbit mAb

(11) Subtype-specific collaborative transcription factor networks are promoted by OCT4 in the progression of prostate cancerNature CommunicationsDecember 1, 2021Ken-ichi Takayama, Takeo Kosaka, Takashi Suzuki, Hiroshi Hongo, Mototsugu Oya, Tetsuya Fujimura, Yutaka Suzuki, Satoshi Inouemonoclonal anti-β-actin (A5441; 1:1000 dilution) was purchased from Sigma. Mouse monoclonal anti-Flag (012-22384; 1:2000 dilution) was purchased from Wako (Tokyo, Japan). Rabbit polyclonal anti-FOXM1(C15410232; 1:500

(12) Copy number-dependent DNA methylation of the Pyricularia oryzae MAGGY retrotransposon is triggered by DNA damageCommunications BiologyDecember 1, 2021Ba Van Vu, Quyet Nguyen, Yuki Kondo-Takeoka, Toshiki Murata, Naoki Kadotani, Giang Thi Nguyen, Takayuki Arazoe, Shuichi Ohsato, Hitoshi Nakayashikiwestern blotting analysis was performed using primary anti-FLAG and anti-HA monoclonal antibodies (Wako, # 018-22381 and # 014-21881), and goat anti-mouse IgG-AP conjugate (Bio-Rad, #1706520) as a secondary antibody.

(13) A novel endoplasmic stress mediator, Kelch domain containing 7B (KLHDC7B), increased Harakiri (HRK) in the SubAB-induced apoptosis signaling pathwayCell death discoveryNovember 19, 2021Yahiro K, Ogura K, Tsutsuki H, Iyoda S, Ohnishi M, Moss J.X-100 in PBS) at room temperature for 1 h. Cells were further incubated with anti-FLAG antibodies (#014-22383, WAKO) in 0.4% BSA/PBS buffer at 4 °C overnight, washed twice with PBS, and incubated with anti-mouse

(14) CSE1L promotes nuclear accumulation of transcriptional coactivator TAZ and enhances invasiveness of human cancer cellsThe Journal of biological chemistryJuly 1, 2021Nagashima S, Maruyama J, Honda K, Kondoh Y, Osada H, Nawa M, Nakahama KI, Ishigami-Yuasa M, Kagechika H, Sugimura H, et al.rabbit polyclonal anti-β-actin (PM053) (Medical and Biological Laboratories Co Ltd); anti-DYKDDDDK-tag (014-22383) and anti-DYKDDDDK-beads (016-22784) (Wako Pure Chemical Industries); rabbit polyclonal anti-mCherry

(15) Overexpression of SARS-CoV-2 protein ORF6 dislocates RAE1 and NUP98 from the nuclear pore complexBiochemical and biophysical research communicationsJanuary 15, 2021Kato K, Ikliptikawati DK, Kobayashi A, Kondo H, Lim K, Hazawa M, Wong RW.(Cell Signaling Technology, 8443), anti-Lamin B1 (MBL, PM064), anti-FLAG (MERCK, F3165; MBL, PM020; and Wako, 018–22381), anti-GFP (Invitrogen, A6455; and Wako, 012–20461), anti-NUP153 (Santa Cruz Biotechnology, sc-101544),

(16) Cytosolic calcium regulates cytoplasmic accumulation of TDP-43 through Calpain-A and Importin α3eLifeDecember 11, 2020Park JH, Chung CG, Park SS, Lee D, Kim KM, Jeong Y, Kim ES, Cho JH, Jeon YM, Shen CJ, et al.(D. melanogaster),UAS-empty,"Park et al., 2020",,SB Lab (DGIST) Antibody,Mouse monoclonal anti-Flag (DYKDDDDK),Wako,Cat#: 012–22384; RRID:AB_10659717,IHC (1:400) Antibody,Rat monoclonal anti-HA,Roche,Cat#: 11867423001; RRID:AB_390918,IHC

(17) The fatty-acid amide hydrolase inhibitor URB597 inhibits MICA/B sheddingScientific ReportsDecember 1, 2020Kazuma Sekiba, Motoyuki Otsuka, Takahiro Seimiya, Eri Tanaka, Kazuyoshi Funato, Yu Miyakawa, Kazuhiko KoikeCell Signaling Technology, Danvers, MA), β-actin (#5125, 1:10,000, Cell Signaling Technology), and FLAG (DYKDDDDK) (#018–22381, 1:1,000, Wako Pure Chemical Industries). Quantitative reverse-transcription PCR (qRT-PCR) Total RNA

(18) Quantifying the dynamics of IRES and cap translation with single-molecule resolution in live cellsNature Structural & Molecular BiologyDecember 1, 2020Amanda Koch, Luis Aguilera, Tatsuya Morisaki, Brian Munsky, Timothy J. StasevichBriefly, immobilized ficin in the presence of 25 mM cysteine was used to digest FLAG antibodies (Wako, 012–22384 Anti DYKDDDDK mouse IgG2b monoclonal) to create Fab. Fab were separated from the Fc region using NAb

(19) SET/TAF1 forms a distance-dependent feedback loop with Aurora B and Bub1 as a tension sensor at centromeresScientific reportsSeptember 24, 2020Asai Y, Matsumura R, Hasumi Y, Susumu H, Nagata K, Watanabe Y, Terada Y.(sc-101013, Santa Cruz Biotechnologies, IB 1:1,000), Histone H2A phospho Thr 12032 (IF 1:50), FLAG (012-22384, Wako, IF 1:1,000 or F7425, Sigma, 1:500), SET/TAF1β50 (KM1720, IF 1:50, IB 1:2,000), hSgo251 (IF 1:2,000

(20) APC/C^Cdh1 is required for the termination of chromosomal passenger complex activity upon mitotic exitJournal of cell scienceSeptember 15, 2020Tsunematsu T, Arakaki R, Kawai H, Ruppert J, Tsuneyama K, Ishimaru N, Earnshaw WC, Pagano M, Kudo Y.Sigma-Aldrich); mouse monoclonal anti-HA, anti-FLAG, anti-c-Myc, and anti-GFP antibodies (#014-21881, #018-22381, #011-21874 and #012-22541, respectively; Wako); rabbit polyclonal anti-survivin antibody (#NB500-201;

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Anti DYKDDDDK tag, Monoclonal Antibody from FUJIFILM Wako Chemicals U.S.A. Corp

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FUJIFILM Wako Chemicals U.S.A. Corp

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Anti DYKDDDDK tag, Monoclonal Antibody from FUJIFILM Wako Chemicals U.S.A. Corp

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