Figure A. Unstimulated cells stained with RPE conjugated Mouse anti Human CD31 (MCA1738PE) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). Figure B. Cells stimulated with TNFα for 19 h then stained with RPE conjugated Mouse anti Human CD31 (MCA1738PE) and Alexa Fluor A700 conjugated Mouse anti Human CD54 (MCA1615A700). All experiments performed on HUVEC cells gated on live single cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer