Fig 2: YWHAB can bind to PIK3R2 in colon cancer cells. (A) Monarch Initiative database predicted that YWHAB can bind to PIK3R2. (B) The mRNA expression of PIK3R2 was detected in colon cancer cell lines by RT-qPCR. (C) The binding of YWHAB and PIK3R2 was verified by co-immunoprecipitation assay. PIK3R2 (D) mRNA and (E) protein expression were detected by RT-qPCR and western blotting. ***P<0.001. YWHAB, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein ß; PIK3R2, PI3K regulatory subunit 2; RT-qPCR, reverse transcription-quantitative PCR; shRNA, short hairpin RNA; NC, negative control.

Fig 3: YWHAB knockdown promotes cell cycle arrest in colon cancer cells at the G0/G1 phase. (A) Cell cycle was detected by PI flow cytometry. (B) The expression of cell cycle markers cyclin D1 and p21 were detected by western blotting. ***P<0.001. YWHAB, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein ß; shRNA, short hairpin RNA; NC, negative control.

Fig 4: YWHAB knockdown promotes cell apoptosis. (A) Cell apoptosis was detected by TUNEL staining. (B) Expression of apoptosis regulator proteins was detected by western blotting. ***P<0.001. YWHAB, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein ß; shRNA, short hairpin RNA; NC, negative control.

Fig 5: YWHAB regulates colon cancer cell apoptosis by binding to PIK3R2. (A) Cell apoptosis was detected using TUNEL staining. (B) The expression of apoptosis regulators was detected by western blotting. ***P<0.001 vs. Control; ##P<0.01 and ###P<0.001 vs. shRNA-YWHAB + Ov-NC. YWHAB, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein ß; PIK3R2, PI3K regulatory subunit 2; shRNA, short hairpin RNA; NC, negative control; Ov, overexpressing vector.