Fig 1: Hepatic inflammation was induced by S. mansoni infection and equally regulated in groups of infected c-Junf/f and infected c-JunΔli animals. (A–D) RT-qPCR demonstrated the induction of Il-1β, Il-4, Il-6, and Il-10, in infected animals compared to non-infected animals, but no differences among infected or ni animals. n = 6 f./f ni, n = 6 Δli ni, n = 11 f./f S.m., n = 12 Δli S.m.; 3 technical replicates each. (E) Western blot analysis and subsequent assessment of optical density of the signals depicted elevated expression for CD45, a marker for leukocytes, in infected animals compared to non-infected animals. n = 6 and 3 technical replicates each. (F) CD45 staining demonstrated CD45-positive granulomatous cells and CD45-positive cells within the parenchyma (red arrows, enlarged in SFig. 11). Both analyses of CD45 expression showed a comparable expression pattern of CD45 in infected c-JunΔli and infected c-Junf/f mice. Magnification 1000 × , bars 100 µm, dashed line granuloma. The indicated p values were calculated by ANOVA and post hoc pairwise comparison of groups using Fisher’s LSD on log transformed data.