Fig 1: PRMT6 protects against alcohol-induced fibrosis. (A–F) Wild-type (WT), Prmt6+/- (Het), and Prmt6-/- (KO) mice were fed western diet (WD, control) or WD with alcohol in the drinking water (WDA, alcohol) for 16 weeks. N = 3–8 mice per group. (A) Examples of liver gross appearance in mice after alcohol feeding. Bars, 0.5 cm. (B, left) Representative images of Sirius red staining. Bars, 200 μm. (Right) Positive area of Sirius red staining, N = 6–10 mice (male and female combined) per group. Area was measured by averaging 3 random images in a blinded manner. ∗∗P < .01. Two-way analysis of variance WT vs KO genotype (F1,32 = 15.37, P = .0004); diet (F1,32 = 14.88, P = .0005); interaction (F1,32 = 4.086, P = .05. (C) Masson trichrome staining of livers from WT and KO mice fed alcohol. Bars, 200 μm. (D) Relative liver mRNA in indicated groups, N = 3–8 mice per group. ∗∗P < .01, ∗∗∗∗P < .001. Two-way analysis of variance Tgfb1 genotype (F2,34 = 5.800, P = .0068); diet (F1,34 = 6.831, P = .0133); interaction (F2,34 = 3.419, P = .04); Col1a1 genotype (F2,25 = 5.814, P = .0084); diet (F1,25 = 22.61, P < .0001); interaction (F2,25 = 1.110, P = .34). (E) Serum PIIINP levels from mice fed alcohol, N = 4. ∗∗P < .01. (F) Prothrombin time (PT) in male mice fed alcohol. N = 5. ∗P < .05.