Fig 1: Expression of selected cell type markers during S and BG differentiation. (A) SWATH-MS results of protein abundances of Ki-67, PCNA, NES, and SOX2 showed a decrease of proliferation and NSC markers, whereas NPC/neuronal marker TUBB3 was increased. Symbols denote significant difference (p < 0.05, moderated t-statistic from limma) of a given group vs. day 0 (#), or day 7 (†), and day 14 (‡) of the same differentiation protocol. (B) Immunodetection of selected NSC, NPC, neuronal, and glial markers. Markers of proliferating cells (Ki-67, PCNA) and undifferentiated NSCs (NES, SOX2) were expressed predominantly in proliferating cells (NSCs, day 0) and decreased during both differentiations. On the other hand, markers of differentiating neurons (TUBB3) and astrocytes (GFAP, S100B) increased during both differentiations, but the expression of astrocyte markers was overall very low. GAPDH and ATPase subunit alpha (ATPa) were used as loading controls. Representative images from two biological replicates are shown.