Fig 2: NOX2 and HK2 induce COL5A1-mediated mesenchymal phenotype in human glioma cells. (a) The levels of COL5A1 and FN1 mRNA and (b) representative immunoblot images (left) and quantification (right) of COL5A1 and FN1 protein levels in human glioma cells treated with NOX2 siRNA or control siRNA. β-actin was used as a loading control. (c) The levels of COL5A1 and FN1 mRNA and (d) representative immunoblot images (left) and quantification (right) of COL5A1 and FN1 protein levels in human glioma cells with NOX2 over-expression (NOX2) or control (Control). β-actin was used as a loading control. (e) Representative images of cellular morphology in U87MG glioma cells with NOX2 over-expression (HK2) or control (Control) (left). Quantification of spindle shape length in U87MG glioma cells with NOX2 over-expression or control (right). Scale bars, 20 μm. (f) The levels of COL5A1 and FN1 mRNA and (g) representative immunoblot images (left) and quantification (right) of COL5A1 and FN1 protein levels in U87MG glioma cells with HK2 over-expression (HK2) or control (Control). (h) Representative images of cellular morphology in U87MG glioma cells with HK2 over-expression (HK2) or control (Control) (left). Scale bars, 20 μm. Quantification of spindle shape length in human glioma cells with HK2 over-expression (HK2) or control (Control) (right). The results are representative of three independent experiments and are shown as the mean ± SEM. * p < 0.05; Student’s two-tailed t-test.