Fig 1: Inflammation induces changes in macrophage subtypes and the development of IUA(A–D) H&E staining (scale bars: 200 µm), Masson’s trichrome staining, and immunohistochemical staining of a-SMA and collagen 1 (scale bars of Masson, a-SMA and Col1: 50 µm) in the endometria of mice after sham operation (sham, n = 8) and on Day 5 (day 5, n = 8), Day 7 (day 7, n = 8) and Day 10 (day 10, n = 8) after modeling.(E) The relative integrated optical density (IOD) was analyzed by Image-pro plus.(F–K) H&E staining (scale bars: 200 µm), immunohistochemical staining of CD45, immunofluorescence analysis of F4/80 (scale bars of CD45 and F4/80: 100 µm), and immunohistochemical staining of CD3, Eomes and CD301 (scale bars of CD3, Eomes and CD301: 50 µm) in the endometria of mice after sham operation (sham, n = 8) and on Day 1 (Day 1, n = 8), Day 2 (Day 2, n = 8), Day 3 (Day 3, n = 8), Day 4 (Day 4, n = 8) and Day 5 (Day 5, n = 8) after modeling.(L) Double immunofluorescence staining of F4/80 (green) and CD301 (red) in the endometria of mice. Scale bars: 50 µm. Arrow: F4/80+ CD301+ macrophage.(M) The mean number of CD45+ leukocytes, F4/80+ macrophages, CD3+ T cells and Eomes+ NK cells per field at a magnification of 200×. Each point represents one mouse.(N) The mean number of CD301+ macrophages per field at a magnification of 400×. Each point represents one mouse. (E) and (M-N) Error bars indicate the mean ± SD. No significant difference (ns), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Supplier Page from Abcam for Anti-TBR2 / Eomes antibody [EPR21950-241]