Fig 1: Overexpression of SOX7 inhibits the proliferation of TSCC cells in vitro. (A) The alteration of SOX7 expression in protein level was identified using western blotting in Cal27 and Tca-8113 cells after transfected with SOX7 vector or NC. (B) Cal27 and (C) Tca-8113 cell viability was detected using MTT assay after overexpression of SOX7. Relative PCNA expression was measured by RT-qPCR in (D) Cal27 and (E) Tca-8113 cells after overexpression of SOX7. Relative clone numbers were measured in (F) Cal27 and (G) Tca-8113 cells by clone formation assay after overexpression of SOX7. SOX7, sex-determining region Y-box 7; TSCC, tongue squamous cell carcinoma; NC, negative control; PCNA, proliferating cell nuclear antigen.*P<0.05, **P<0.01.
Fig 2: miR-184 is significantly increased and SOX7 was decreased in the TSCC cell lines and tissues. (A) miR-184 expression levels in TSCC cell lines and normal cell line were tested using RT-qPCR. The expression levels were calculated using miR-184/U6 expression ratio (2−ΔΔCq). (B) The expression levels of miR-184 in TSCC and the corresponding non-tumorous tissues were identified using RT-qPCR. (C) SOX7 expression levels in TSCC cell lines and normal cell line were tested using the RT-qPCR. (D) The expression levels of SOX7 in TSCC and the corresponding non-tumorous tissues were identified using RT-qPCR. (E) The correlation between miR-184 and SOX7 expression was negative (r=−0.7373, p<0.0001). **P<0.01. SOX7, sex-determining region Y-box 7; TSCC, tongue squamous cell carcinoma.
Fig 3: SOX7 is the direct target of miR-184 and miR-184 regulates the expression of SOX7. (A) The binding site of SOX7 and miR-184 was predicted using the TargetScan tool. (B) Cal27 cells were transfected with the p-MIR-SOX7-3′-UTR-WT or p-MIR-SOX7-3′-UTR-MT luciferase reporter and miR-184 mimic. The luciferase activity was measured using the ONE-Glo Luciferase Assay instrument. Relative SOX7 expression was tested in (C) Cal27 and (D) Tca-8113 cells after transfected with miR-184 mimic or inhibitor. **p<0.01; ##p<0.01. SOX7, sex-determining region Y-box 7.
Supplier Page from Abcam for Anti-SOX7 antibody