Fig 1: NR2F1-AS1 upregulates ST8SIA1 expression by recruiting SPI1. (a) lncRNA Modulator Atlas in Pan-cancer database was used to determine that NR2F1-AS1 bound with ST8SIA1 by recruiting SPI1. Relative (b) protein and (c) mRNA expression levels of ST8SIA1 in GES-1 and AGS cells were detected using Western blotting and RT-qPCR, respectively. Relative (d) protein and (e) mRNA expression levels were detected of ST8SIA1 in the control and transfection group using Western blotting and RT-qPCR, respectively. (f) shRNA-NR2F1-AS1 co-transfected with the pGL3-ST8SIA1-WT/MUT luciferase reporter was used to confirm the binding relationship between NR2F1-AS1 and ST8SIA1. (g) RNA pull-down and (h) RNA immunoprecipitation assays were performed to detect the binding relationship between NR2F1-AS1 and SPI1. (i) Chromatin immunoprecipitation assay was performed to further verify the binding between ST8SIA1 and SPI1. *P < 0.05, **P < 0.01, ***P < 0.001 vs. GES-1, shRNA-NC or IgG
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