Fig 1: Sox11 ameliorates reduced cellular viability and proliferation induced by OGD/R. (A) Verification of Sox11 overexpression, as shown by qRT-PCR. (B) Verification of Sox11 overexpression, as shown by western blotting. (C) Quantitation of Sox11 protein levels in the different groups. (D) Cell viabilities in the different groups, as shown by the CCK-8 assays. (E) Cell proliferation in the different groups, shown by the EdU staining assay (scale bar, 50 µm). For statistical analysis, different letters showed significant differences between groups in SH-N-SH or SH-SY5Y cells. OGD/R, oxygen-glucose deprivation/reoxygenation; qRT-PCR, quantitative real-time polymerase chain reaction; CCK-8, Cell Counting Kit-8; OE, overexpression; EdU, 5-ethynyl-2'-deoxyuridine; DAPI, 2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride.
Fig 2: Dex positively regulates the expression of Sox11 under OGD/R conditions. (A) The relative Sox11 mRNA expressions in the different groups measured by qRT-PCR. (B) Sox11 protein levels evaluated by western blotting. (C) Quantitation of Sox11 protein levels in the different groups. For statistical analysis, different letters showed significant differences between groups in SH-N-SH or SH-SY5Y cells. OGD/R, oxygen-glucose deprivation/reoxygenation; Dex, dexmedetomidine; qRT-PCR, quantitative real-time polymerase chain reaction.
Fig 3: Dex ameliorates OGD/R-mediated cell damage via up-regulation of Sox11. (A) The efficacy of Sox11 knockdown verified by western blotting. (B) Cell viability in the different groups, as determined by CCK-8 assays. (C) Quantitation of proliferation in the different groups, as determined by EdU assays. (D) Quantitation of apoptosis in the different groups, as measured by flow cytometry. (E) Levels of apoptosis-related proteins in the different groups, as shown by western blotting. For statistical analysis, different letters showed significant differences between groups in SH-N-SH or SH-SY5Y cells. Dex, dexmedetomidine; OGD/R, oxygen-glucose deprivation/reoxygenation; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2'-deoxyuridine; sh, short hairpin RNA; CTRL, control.
Fig 4: Sox11 reduces apoptosis induced by OGD/R. (A) Apoptosis ratios for the different groups, as measured by flow cytometry. (B) Quantitation of apoptosis in the different groups, as measured by flow cytometry. (C) Apoptosis-related proteins in the different groups, as shown by western blotting. (D) Quantitation of apoptosis-related in the different groups. For statistical analysis, different letters showed significant differences between groups in SH-N-SH or SH-SY5Y cells. OGD/R, oxygen-glucose deprivation/reoxygenation; OE, overexpression; FITC, fluorescein isothiocyanate; PI, propidium iodide; CTRL, control.
Supplier Page from Abcam for Anti-SOX11 antibody