Fig 1: The effects of OIP5-AS1/miR-195-5p/NOB1 pathway on HemECs cell migration, invasion and apoptosis. (A,B) The proliferative rate of HemECs was detected by MTT and colony formation assays. (C) The apoptotic rate was examined by flow cytometry assay. (D,E) The migration and invasion of HemECs cells were detected by Transwell assay. Bar = 200 μm. (F) The vasoformation of HemECs cells was examined by tube formation assay. ∗P < 0.05 and ∗∗P < 0.01 vs. controls.
Fig 2: OIP5-AS1 regulates NOB1 through binding to miR-195-5p. (A) The binding sites between miR-195-5p and NOB1 were achieved by bioinformatics analysis. (B) The relation between miR-195-5p and NOB1 was conducted by pull-down assay. (C) The luciferase activity of NOB1 could be affected by miR-195-5p mimics. (D) The expression of NOB1 was detected in different tissues. (E,F) The correlations between miR-195-5p/NOB1 and OIP5-AS1/NOB1 were detected by Spearman’s analysis (n = 56). (G,H) Expression of NOB1 after miR-195-5p mimics and pcDNA-OIP5-AS1 as well as sh-OIP5-AS1 and miR-195-5p inhibitors were transfected into HemECs. ∗P < 0.05 and ∗∗P < 0.01 vs. controls.
Fig 3: Validation of the expressions of heat shock protein 1B, NOB1, BCLAF1 and CRIP1 by IHC. Representative images of IHC analysis of the four proteins in HT and NC group were shown. The scale bar is 50 μm. HT=high temperature (45° C), NC=negative control (37° C).
Supplier Page from Abcam for Anti-NOB1 antibody