Fig 1: MO downregulates STEP by modulating the PP2B/DARPP-32/PP1 axis. (A) The expression level of DARPP-32, p-T34, PP1, and p-T320 from hippocampal lysates of WT control and APP/PS1 mice with or without MO treatment was evaluated by Western blotting, (n = 3). (B–E) The statistical analysis of Western blot bands from panel A. ß-actin serves as the loading control. (F) The statistical analysis of the PP2B activity test from hippocampal lysates of WT control and APP/PS1 mice with or without MO treatment, (n = 4). The test was carried out using a PP2B activity kit. The data are presented as Mean ± SEM. ** p < 0.01 and **** p < 0.0001 vs. WT or vs. APP/PS1 mice groups. p-T34 = phosphorylated DARPP-32 at Thr34; p-T320 = phosphorylated PP1 at Thr320.