Fig 2: SOCS7 regulates HGSOC cell growth both in vitro and in vivo. a The mRNA and protein levels of SOCS7 in various HGSOC cell lines and human ovarian epithelial cell line IOSE80. b Association between SOCS7 expression and cell cycle, based on GSEA analysis. c-e SOCS7 overexpression and knockdown in HGSOC cells transduced with indicated lentiviral vectors. f–h Viability and i-n cell cycle of HGSOC cells transduced with indicated lentiviral vectors. o-r CAOV3 cells transduced with pLVX-Puro-SOCS7 or blank pLVX-Puro were injected into nude mice, and SOCS7 expression levels (o), tumor volume (p), weight (q), and the immunofluorescence staining for Ki67 (r) were determined. Scale bar = 50 µm. *P < 0.05, **P < 0.01, and ***P < 0.001, in related with IOSE80, shNC or Vector

Fig 3: HuR binds to the 3'UTR of FOXM1 and regulates its transcription. a and b Pearson's correlation scatter plots for FOXM1-SOCS7 (a) and FOXM1-HuR (b) of HGSOC patients (n = 30) in cohort 1. c Additional HuR RIPs followed by qRT-PCR on precipitates validated HuR binding of FOXM1 mRNA in CAOV3 cells transduced with indicated lentiviral vectors. d Relative luciferase activity of FOXM1 3'UTR in CAOV3 cells transduced with indicated lentiviral vectors. e Relative mRNA levels of FOXM1 in CAOV3 cells transduced with indicated lentiviral vectors and treated with 5 µg/ml actinomycin D. f-i HuR and FOXM1 expression in CAOV3 cells transduced with indicated lentiviral vectors. *P < 0.05, **P < 0.01 and ***P < 0.001, in related with shNC, Vector. ###P < 0.001, in related with SOCS7

Fig 4: HuR is involved in the regulation of HGSOC cell viability and cell cycle progression by SOCS7. a HuR overexpression in CAOV3 cells. b Viability, c and d cell cycle distribution of CAOV3 cells transduced with indicated lentiviral vectors. e HuR protein expression in CAOV3 cells transduced with indicated lentiviral vectors. **P < 0.01, ***P < 0.001, in related with Vector. ###P < 0.001, in related with SOCS7

Fig 5: SOCS7 was a direct target of miR-199a-5p.(A) Schematic representation of the miR-199a-5p site in SOCS7-3' UTR. (B) The expression of miR-199a-5p was assessed using qPCR analysis in LX2 cells transfected with miRcont inhibitor or miR-199a-5p mimic. (C) Luciferase activity was assayed in LX2 cells cotransfected with miR-199a-5p and luciferase reporters containing SOCS7-3' UTR. Data are presented as the relative ratio of firefly luciferase activity to Renilla luciferase activity. (D) The mRNA expression of SOCS7 was assessed using qPCR analysis in LX2 cells transfected with NC or miR-199a-5p mimic. (E and F) Representative western blot of SOCS7 protein expression in LX2 cells transfected with miRcont or miR-199a-5p mimic, and quantification of average across three separate experiments. * P < 0.05, *** P < 0.001.