Fig 2: PCAT6 promoted PCa growth and metastasis in vivo. (A, B) Cancer metastasis was assayed using bioluminescent imaging after PCAT6-overexpressed. (C, D) LNCaP cells stably expressed with PCAT6 were injected intravenously into the tail vein of male nude mice and tumor volume was calculated at the different time points (7, 14, 28, 35 days). (E) qPCR analysis of PCAT6, NSE, SYP, and ChgA mRNA expression in LNCaP cells after PCAT6 overexpression. (F) Western blot analysis for NSE, SYP, and ChgA protein level in LNCaP cells after PCAT6 overexpression. **p<0.01.

Fig 3: The role of PCAT6/miR-326/Hnrnpa2b1 axis in regulating PCa cells proliferation, invasion, and NED. (A) qPCR analysis of NSE, SYP, and ChgA mRNA expression in LNCaP cells after PCAT6 overexpression in the presence or absence of miR-326 overexpression or Hnrnpa2b1 knockdown. (B, C) Western blot analysis for NSE, SYP, and ChgA protein level in LNCaP cells after PCAT6 overexpression in the presence or absence of miR-326 overexpression or Hnrnpa2b1 knockdown. (D) LNCaP cell proliferation was assayed using CCK-8 after PCAT6 overexpression in the presence or absence of miR-326 overexpression or Hnrnpa2b1 knockdown. (E, F) LNCaP cell invasion was assessed using transwell invasion assay after PCAT6 overexpression in the presence or absence of miR-326 overexpression or Hnrnpa2b1 knockdown. **p<0.01.