Fig 2: vWF protein expression of the differentiating MSCs. (A) Certain AMSCs began to express vWF protein after culture in DMEM containing VEGF (50 ng/ml) for 5 days (magnification, x100; scale bar, 100 µm); (B) magnified window of A (magnification, x200; scale bar, 50 µm). (C) Certain GMSCs began to express vWF protein after culture in DMEM containing VEGF (50 ng/ml) for 5 days (magnification, x100; scale bar, 100 µm); (D) magnified window of C (magnification, x200; scale bar, 50 µm). Staining for vWF is in red and nuclei are counterstained with DAPI (blue). (E) Quantitative results. Values are presented as the mean ± standard deviation. *P<0.05 vs. AMSCs. MSCs, mesenchymal stem cells; vWF, von Willebrand factor; AMSCs, adipose MSCs; GMSCs, gingival MSCs.

Fig 3: Cold storage promotes CIRP secretion in Kupffer cells. (A) Immunohistochemical staining of CIRP, CD68, and vWF in DCD livers, which were in cold storage for 1 h and 4 h (magnification 200×). (B) The number of CIRP‐positive cells in the CS4h group was significantly higher than in the CS1h group (n = 6). (C) The number of CD68‐positive cells in the CS4h group was higher than in the CS1h group. (D) There was no difference between the two groups for the number of vWF‐positive cells (p = 0.277). (E) Kupffer cells cultured in a hypothermic/hypoxia environment had upregulated expression of CIRP and TLR‐4 compared to cells cultured in a normal environment. *p < 0.05 versus CS1h. Scale bars: 50 μm (A)