Fig 1: SILAC-based pull-down experiments with immobilized peptide containing three NARR repeats. (A) Sequence of the peptide used for the pulldown. (B) ERF1 and (C) EIF5A were found with SILAC ratios indicating specific binding. Representative spectra of EIF5A, ERF1 and UBF peptides obtained by the SILAC-based profiling are shown in corresponding boxes. Asterisks and diamonds indicate relative amount of proteins bound to the NARR-peptide column and control column, respectively. The interactions were confirmed by western blotting. (D) UBF does not specifically interact with the NARR peptide. (E) Boxplot of the results of the SILAC-based pull-down experiment indicates the interactions of EIF5A and ERF1 with the NARR peptide.
Supplier Page from MilliporeSigma for Anti-eRF1 antibody produced in rabbit