Fig 1: GDM-hAMSCs exhibit a specific pro-angiogenic expression profile. A Heatmap comparing angiogenic gene expression between the GDM and NGT groups (N = 14 in each). Data are represented as log2 (fold change). Differences assessed by Mann–Whitney U test. B Western blot analysis of FGFR2, TGFBR1, PAI-1 and VEGFA protein expression. Representative image and densitometry analysis (arbitrary units, N = 9 each group). Data are normalized to the mean of the controls of each experiment and are shown as mean ± SEM. Differences assessed by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001
Fig 2: GDM-like milieu confers a pro-angiogenic profile on NGT-hAMSCs. NGT-hAMSCs were cultured in the presence of 30 mM glucose, 100 nM insulin and 50 µM palmitic acid. A Visual representation of tube formation assay of NGT-hAMSCs and GDM-like treated hAMSCs. Two representative fields of each condition are shown. Scale bars = 100 µm B Differences in the number of extremities, nodes, junctions and meshes between groups (N = 10 each group). C Protein expression analysis comparing angiogenic protein levels of FGFR2, TGFBR1, PAI-1 and VEGFA between groups. Representative image and densitometry analysis (arbitrary units, N = 9 each group). Data are normalized to the mean of the controls of each experiment and are shown as mean ± SEM. Differences assessed by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001
Supplier Page from MilliporeSigma for Anti-TGFBR1 (center) antibody produced in rabbit