Fig 1: TSC1 depletion in macrophage aggravated mice IR-induced tubular injury. (A) Schematic diagram. (B) Serum creatinine levels in wild-type (WT) and Lyz-TSC1 cKO mice at baseline (0) and 1 and 4 days post-IRI. (C) Representative H&E-stained renal sections of WT and Lyz-TSC1 cKO mice. Scale bar: 20 µm. (D) Quantification of H&E staining corresponding to the WT and Lyz-TSC1 cKO mice shown in (C). (E) Representative Kidney Injury Molecule 1 (KIM-1) immunohistochemistry in renal sections from WT and KO mice on day 1 post-IRI (×200). (F) Quantification of KIM-1 immunohistochemistry-stained murine renal cortical sections on day 0 and day 1 after IRI. In (B, D, F), the data are shown as the mean ± SD and were analyzed by an unpaired two-tailed Student’s t-test (n = 3). *P < 0.05; **P < 0.01; NS, not significant.
Fig 2: Dynamic changes of CPT1a during kidney injury and repair in folic acid nephropathy model.Changes of BUN (A) and creatinine (B) in groups as indicated. n = 5 for each group. * P < 0.05 versus control. C Representative images of mice kidney samples from groups as indicated stained with Masson trichrome and Sirius red. D Percentage of the fibrotic area in groups as indicated. n = 5-8 for each group. * P < 0.05 versus control. E Representative images of mice kidney samples immunostained with KIM-1. F Western blot analysis of protein expression of KIM-1 and NGAL in kidney samples from groups as indicated. G Western blot analysis of protein expression of CPT1a in kidney samples from groups as indicated. H Graphic presentation of the relative abundance of CPT1a in the folic acid model. I Representative images of mice kidney samples immunostained with CPT1a. Scale bar, 50 µm.
Fig 3: Genetic ablation of CPT1a aggravates tubular cell injury and hampers kidney repair.A Western blot analysis of protein expression of CPT1a in kidney samples from groups as indicated. Changes of BUN (B) and creatinine (C) in groups as indicated. n = 8 for each group. D Representative images of mice kidney samples from groups as indicated stained with Masson trichrome and Sirius red. E Percentage of the fibrotic area in groups as indicated. n = 6 for each group. * P < 0.05 versus control. # P < 0.05 versus CPT1a+/+. F Representative images of mice kidney samples immunostained with KIM-1. G Western blot analysis of protein expression of KIM-1 and NGAL in kidney samples from groups as indicated. Scale bar, 50 µm.
Supplier Page from MilliporeSigma for Anti-TIM-1 antibody produced in rabbit