Fig 2: Release of pro-inflammatory mediators in AML-193 cells inhibited by biochanin A in coculture of A427 and AML-193 cell lines. Various concentrations of biochanin A were pretreated with A427 cell lines for 24 h and cocultured with AML-193 cells at a ratio of 1:15. Treatment of biochanin A resulted in concentration-based inhibition of TNF-α (A) and IL-6 (B) as compared with the blank control and DMSO controls. Data are represented as mean + standard deviation of three independent experiments. *P<0.05; **P<0.01 vs. Control. TNF, tumor necrosis factor; IL, interleukin.