Fig 1: Immunohistochemical localization of encoded proteins for several differentially expressed genes. Protein immunoreactivity was visualized as a dark purple precipitate. (a) TH, (b) DAT, (c) FOXA2, (d) MRAP2, (e) CCL2, (f) SERPINA3, (g) CDKN1A, (h) GADD45B, (i) FOS, and (j) EGR1. Note the prominent labeling of DA neurons, also readily identifiable by their characteristic large nuclei and intracellular neuromelanin accumulation (brown). Scale bars=20 µm.