Fig 1: YAP binds YREs in loci of melanocytic transcription factors in Uveal Melanoma.a Genome browser snapshot of the SOX10 locus, showing YMCi-13K screen results (scoring regions and Log2-fold change at day 22 using bigwig tracks with 250 bp span), HiC data, HiChIP-H3K27ac significant loops (FDR < 0.05), Cut&Tag tracks for YAP1, PAX3, SOX10, MITF, TFAP2A and CTCF, and ChIP-seq tracks for H3K27ac, H3K4me1, H3K4me3 and the RNA polymerase II subunit RPB1. Scoring regions validated in single assays are color-shaded in purple. b Violin plots showing Cut&Tag signal coverage of indicated marks/factors (H3K27ac, YAP1, PAX3, SOX10 or MITF) in the group of regions that commonly score in all cell lines (gray color), or that score specifically in mesothelioma (green color) or uveal melanoma (purple color). Horizontal line in violin plot represents median of data distribution. Significance was assessed by two-sided Wilcoxon test (*P < 0.05; **P < 0.01; ***P < 0.001, and not significant (n.s) P > 0.05). Source data are provided as a Source Data file. c Model depicting the findings of the study. YAP engages lineage-specific YREs to establish feedforward loops with specific transcription factors critical for lineage-specific cell proliferation.
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