Fig 1: M. tuberculosis modulates NRs expression in murine macrophages and dendritic cells. NRs undergoing change in expression in both mBMDMs and mBMDCs are plotted. Comparative expression analysis of (A–J) NRs in mBMDMs and mBMDCs at 0 h, 12 h and 48 h post H37Rv infection. (K) A schematic representation showing NRs with significantly similar and opposite expression pattern in mBMDMs and mBMDCs at 0 h, 12 h and 48 h after H37Rv infection. (L) Immunoblot analysis of Nr4a3 and Rora in H37Rv infected mBMDCs and mBMDMs at 0 h, 12 h and 48 h post H37Rv infection. β-actin was used as loading control. The full length or source images of all immunoblots have been provided in the supplementary information. Results are mean and s.d. (A–J) or representative (L) of three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001, versus uninfected mBMDMs or mBMDCs (one-way ANOVA).
Fig 2: Expression analysis of Nr4a3 and Rora in hMDMs and clinical samples. (A) RT-qPCR analysis for relative expression of Nr4a3 and Rora in H37Rv infected hMDMs isolated from healthy individuals at 0 h, 12 h and 48 h post infection. Results are mean and s.d. of three independent experiments. *p < 0.05, versus uninfected (one-way ANOVA). (B) Relative mRNA abundance of Nr4a3 and Rora in human PBMCs isolated from freshly diagnosed TB patients (n = 10) was calculated as 2−(ΔΔCt) relative to healthy individuals (n = 10).
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