Fig 1: RXFP3 expression in GABA neurons in the VTA. a, Left panel shows ISH image from the Allen Mouse Brain Atlas showing mosaic expression of RORA in the VTA and SNC. b, Graph showing mean (+SEM) percentage of RXFP3+ cells (n = 442 cells) that express either HA, or TH, or HA and TH, or neither. c, Graph showing mean (+SEM) percentage of HA+ cells (n = 526) that express RXFP3 and the percentage of TH+ cells (n = 394 cells) that express RXFP3. d, e, Representative examples of immunolabelling of RXFP3 (magenta), HA (blue) and TH (green) in the VTA of a VGATCre:RiboTag mouse, illustrating exemplar cells exhibiting RXFP3 co-localisation with HA, but not TH (yellow arrows). f, Representative example of immunolabelling in the SNc. [Colour figure can be viewed at http://www.wileyonlinelibrary.com]
Fig 2: RORA expression in GABA neurons in the VTA. a, Left panel shows ISH image from the Allen Mouse Brain Atlas showing mosaic expression of RORA in the VTA and SNC. b, Graph showing mean (+SEM) percentage of RORA+ cells (n = 250 cells) that express either HA, or TH, or HA and TH, or neither. c, Graph showing mean (+SEM) percentage of HA+ cells (n = 367) that express RORA and the percentage of TH+ cells (n = 244 cells) that express RORA. d, e, Representative examples of immunolabelling of RORA (magenta), HA (blue) and TH (green) in the VTA of a VGATCre:RiboTag mouse, illustrating exemplar cells exhibiting RORA co-localisation with HA, but not TH (yellow arrows). f, Representative example of immunolabelling in the SNc. [Colour figure can be viewed at http://www.wileyonlinelibrary.com]
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