Fig 1: The hippocampal protein levels of Arc and Syt1 in CD-1 mice. (A) Representative photomicrographs of Arc and Syt1 protein immunolabeling in the dorsal hippocampus and its subfields in 15-month-old (15 M) LPS + S-treated mice (top row is the dorsal hippocampus, low magnification; second to fourth rows represent different subregions, high magnification). (B) Representative Arc and Syt1 immunoreactive bands in the hippocampi of mice in the different treatment groups at different ages. (C,E) Arc and (D,F) Syt1 protein levels in the hippocampi of mice of different ages in the different treatment groups. Scale bar = 200 µm at low magnification and 50 µm at high magnification. Error bars = SEM. *P < 0.05, **P < 0.01 compared with the control group; $P < 0.05, $$P < 0.01 compared with the CON + S group; #P < 0.05, ##P < 0.01 compared with the LPS group.
Fig 2: The levels of Arc and Syt1 mRNA in different hippocampal subregions in CD-1 mice. (A,B) Arc mRNA, and (C,D) Syt1 mRNA levels in different hippocampal subregions (CA1, CA3, and DG) in the different treatment groups at 3 months (3 M) (A,C) and 15 months (15 M) (B,D) of age. Error bars = SEM. *P < 0.05, **P < 0.01 compared with the control group; $P < 0.05, $$P < 0.01 compared with the CON + S group; #P < 0.05, ##P < 0.01 compared with the LPS group. CA, cornu ammonis; DG, dentate gyrus; CON, untreated control group; LPS, lipopolysaccharide treatment group; S, group of mice exposed to stress.
Fig 3: Representative immunoreactive bands for Arc and Syt1 (a) in the hippocampi of mice in different treatment groups at different ages. (b) Arc and (c) Syt1 protein levels in the hippocampi of mice at different ages in the different treatment groups (n = 6 per group). Error bars = SEM. *P < 0.05, **P < 0.01 compared with the control group; ##P < 0.01 compared with the LPS group. CON: untreated control group; LPS: lipopolysaccharide treatment group; E: group of mice exposed to EE.
Fig 4: The expression levels of Arc and Syt1 mRNA in different hippocampal subregions in CD-1 mice. (a) Arc mRNA and (d) Syt1 mRNA levels in different hippocampal subregions (CA1, CA3, and DG) at different ages in the control groups. (b, c) Arc mRNA and (e, f) Syt1 mRNA levels in different hippocampal subregions (CA1, CA3, and DG) in the different treatment groups (n = 6 per group) at 3 months of age (b, e) and 15 months of age (c, f). The relative mRNA level was calculated as the number of positive cells expressing mRNA. Error bars = SEM. *P < 0.05, **P < 0.01 compared with the control group; #P < 0.05, ##P < 0.01 compared with the LPS group. CON: untreated control group; LPS: lipopolysaccharide treatment group; E: group of mice exposed to EE; MWM: Morris water maze.
Fig 5: Representative photomicrographs of Arc and Syt1 mRNA levels in different hippocampal subregions in CD-1 mice at different ages and under different treatments. Scale bar = 20 µm in the CA1 and CA3, 50 µm in the DG. CA: cornu ammonis; DG: dentate gyrus.
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