Fig 1: FOXG1 inhibits the apoptosis of NPC cells. (A) Flow cytometry analysis was used to detect the apoptosis of C666-1 and SUNE-1 cells. (B) Western blot analysis was conducted to detect the expression levels of apoptosis-related proteins (Bax, Bcl-2, PARP, cleaved PARP, caspase-3, cleaved caspase-3, caspase-8, cleaved caspase-8, cleaved caspase-9 and caspase-9) in C666-1 and SUNE-1 cells. **P<0.01 vs. Control, si-NC or pcDNA3.1-NC group. NC, negative control; si, small interfering RNA; FOXG1, forkhead-box gene 1; PARP, poly(ADP-ribose) polymerase 1.
Fig 2: Effect of vanillin on caspase 3 and PARP1 activation. (A) Western blot analysis of cleaved caspase-3 (CC3) (A), PARP1 and cleaved PARP1 (c-PARP) (B) in H9c2 cells exposed to 20-µM doxo for 24 h, pretreated (Pretr) and cotreated (Cotr) with 100-µM vanillin. UT—untreated cells; doxo—cells exposed to 20-µM doxo; Van—cells exposed to 100-µM Van. Images are representative of western blotting from two different cellular extract with similar results. Data represent the average of three independent experiments ± SD. **: p < 0.01; ***: p < 0.001 by one-way ANOVA. Other experimental details are described in Materials and Methods section.
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