Fig 1: Modulating the coupling between H2O2 and matrix glutathione links matrix glutathione oxidation to cell death Hydrogen peroxide “acute stress” assay. Δprx1 cells co‐transformed with an empty plasmid or a plasmid encoding Prx1, Tsa1, PRDX3, PRDX5, PRDX6, PfAOP, or PfAOP‐L109M were grown in SD medium lacking the appropriate amino acids for plasmid selection and pre‐treated with 0, 1, 5, 10, and 25 mM H2O2 for 30 min. Afterward, the cells were diluted, and a fixed volume was plated on YPD plates. The number of viable colonies was counted after 2 days of growth at 30°C, here represented as a percentage relative to the 0 mM pre‐treatment. Error bars represent standard deviation (n = 3–8 biological replicates, with cells taken from independent cultures for each individual biological replicate). Significance was assessed with Student's 2‐tailed, unpaired, t‐test. *P < 0.05; **P < 0.01; and ***P < 0.001.The efficiency of transfer of oxidation from H2O2 to E GSH strongly correlates with cell death upon exposure to acute H2O2 stress. Data from viability and matrix Grx1‐roGFP2 responses during acute H2O2 stress were correlated.
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