Fig 1: PALM2 interacts with ezrin.A, left, Coomassie blue staining of the IP samples before the mass spectrometry analysis; right, interacting-protein network of PALM2 in the BioGRID database. B, PALM2 interacted with ezrin, but PALM2C408S did not. HEK 293T cells were co-transfected with GFP-EZR and Flag-PALM2 or PALM2C408S and following Flag IP, samples were characterized by Western blotting with antibodies against Flag and GFP. C, FTI 277, FTase inhibitor, suppressed the interaction between PALM2 and ezrin. After HEK 293T cells were transfected with Flag-PALM2 for 12 h, FTI 277 was added for 48 h, and following Flag IP, samples were characterized by Western blotting with antibodies against Flag and ezrin. D, sketch map of ezrin and its truncations. E, PALM2 interacted with the FERM domain of ezrin. Cells were co-transfected with Flag-PALM2, and the different truncated types of ezrin, and then GFP IP samples were characterized by Western blotting with antibodies against Flag and GFP. Blue stars indicate GFP-vector, GFP-EZR, GFP-EZR1-467 aa, GFP-EZR1-316 aa, and GFP-EZR317-586 aa proteins. F, PALM2 interacts with K253/254/262/263 in the ezrin FERM domain. Cells were co-transfected with Flag-PALM2, and the mutant ezrins, and then GFP IP samples were characterized by Western blotting with antibodies against Flag and GFP. Blue stars indicate GFP vector.