Fig 1: Effects of osmotin and silencing of AdipoRs on expressions of proinflammatory factors in H9c2 cells. H9c2 cells were pre-treated with OGD/R, and then been administrated with osmotin and/or transfection with specifically siRNAs for AdipoR1 or AdipoR2. (A) Expressions of proinflammatory factors including IL-1β, IL-6, IL-8, and TNF-αmeasured by western blot. (B–E), Relative protein expression levels of proinflammatory factors IL-1β, IL-6, IL-8, and TNF-α(n = 5). *P < 0.05 compare to corresponding control; ns, no significant compared to the osmotin group. OGD/R, oxygen and glucose deprivation/reperfusion; AdipoR, adiponectin receptor; IL, interleukin; TNF, tumor necrosis factor; NF-κB, nuclear factor-kappa B; siRNA, small interfering RNA; ns, non-significant.
Fig 2: Effects of osmotin and silencing of AdipoRs on inflammatory factors in H9c2 cells. H9c2 cells were pre-treated with OGD/R, and then been administrated with osmotin and/or transfection with specifically siRNAs for AdipoR1 or AdipoR2. (A–D). The relative contents of proinflammatory factors IL-1ß, IL-6, IL-8, and TNF-a were measured by ELISA assay (n = 5). (E) Protein expressions of anti-inflammatory factors IL-4, IL-10 and IL-13 were measured by western blot. *P < 0.05 compare to corresponding control; ns, no significant compared to the osmotin group. OGD/R, oxygen and glucose deprivation/reperfusion; AdipoR, adiponectin receptor; IL, interleukin; TNF, tumor necrosis factor; siRNA, small interfering RNA; ns, non-significant.
Fig 3: Effects of osmotin and administration of LY294002 on the proinflammatory factors expressions in H9c2 cells. H9c2 cells were pre-treated with OGD/R, and then been administrated with osmotin and/or LY294002 as inhibitor of PI3K. (A) Expressions of IL-1β, IL-6, IL-8, and TNF-α in H9c2 cells after administration were measured by western blot. (B–E) Relative expression levels of IL-1β, IL-6, IL-8, and TNF-α(n = 5). *P < 0.05 compare to corresponding control. OGD/R, oxygen and glucose deprivation/reperfusion; LY294002, inhibitor of phosphatidylinositol 3-kinase (PI3K); IL, interleukin; TNF, tumor necrosis factor.
Fig 4: Effects of osmotin and administration of LY294002 on the inflammatory factors contents in H9c2 cells. H9c2 cells were pre-treated with OGD/R, and then been administrated with osmotin and/or LY294002 as inhibitor of PI3K. (A–D) Results of ELISA assay about the contents of proinflammatory factors IL-1β, IL-6, IL-8, and TNF-α(n = 5). (E) Protein expressions of anti-inflammatory factors IL-4, IL-10 and IL-13 were detected by western blot *P < 0.05 compare to corresponding control. OGD/R, oxygen and glucose deprivation/reperfusion; AdipoR, adiponectin receptor; LY294002, inhibitor of phosphatidylinositol 3-kinase (PI3K); IL, interleukin; TNF, tumor necrosis factor.
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