Fig 1: OL nucleomegaly and apoptosis in white matter of Aze-treated adult mice (Experiment A1). (A) Diffuse enlargement and clearing of nucleoplasm in OL in cerebellar white matter in Aze-treated mice as compared to those in a saline-treated control. Arrows indicate areas shown at higher power in the insets. H&E, original magnification: 240×. (B) OL nuclear diameters in Aze-treated and control mice. Numbers of OL nuclear measurements are indicated. *p<0.001, One-way ANOVA. (C) Immunostains for the OL-specific marker CNPase highlight nuclear swelling (black arrows) and pyknotic (possibly apoptotic), nuclei (red arrows) in the Aze-treated but not the control-treated mouse spinal cord white matter (WM). OL nuclear swelling is not evident in the gray matter (GM). No effects of Aze on GM neurons are evident. Original magnification: 240×.
Fig 2: Ectopic white-matter bundles in the anterior cingulate cortex (ACC) express 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and lack synaptic antigens. Immunostaining of CNPase and synaptic markers in the ACC of representative (A, C, E, G) BTBR and (B, D, F, H) B6 brains. The images are nearly adjacent to those shown in Figure 3. (A) Focal white-matter bundles had strong immunoreactivity for CNPase, a marker of oligodendrocytes and myelinated fibers. An absence of markers of pre and postsynaptic antigens, as revealed by (C) drebrin (E) and vesicular glutamate transporter VGluT1 and (G) synaptophysin immunoreactivities, was seen in the anterior cingulate cortex of BTBR brain. (D, F, H) Staining with synaptic markers appeared to be uniform in the corresponding cortical region from B6 mice. Scale bar = 25 µm.
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