Fig 1: Effect of luzindole or 4P-PDOT on melatonin-induced lipolytic changes in BIPs. (A) Effect of luzindole and 4P-PDOT on melatonin-induced adipocyte lipolysis; (B) BIPs differentiated over 8 days were incubated in fresh medium with or without 10 μM isoproterenol; (C–E) the mRNA expression of lipolytic related genes (ATGL, HSL, and PLIN1); (F) protein level of HSL was quantitated using densitometry and normalized to β-actin levels. The medium was collected to detect the glycerol release, a measure of lipolysis, using the Adipolysis Assay Kit. Lipolysis was calculated in nmol per mg total protein per h. The mRNA expressions were normalized to housekeeping genes β-actin and GAPDH. Values are presented as the means ± S.E.M. “*”represents significant differences, P < 0.05; “**”represents significant differences P < 0.01. 4P-PDOT = 4-phenyl-2-propionamidotetraline; BIPs = bovine intramuscular preadipocytes; HSL = hormone sensitive lipase; ATGL = adipocyte triglyceride lipase; PLIN1 = perilipin 1.
Supplier Page from MilliporeSigma for Anti-HSL antibody produced in rabbit