Fig 1: Effect of Rg1 on sphingolipid pathway proteins. (A) SPHK1, SGPL1, p-Akt, p-Erk1/2, Akt and Erk1/2 expression levels were detected in HHL-5 cells treated with Rg1 (0.2, 0.4 and 0.6 mM) after exposure to MCE (1%) by western blotting. (B) SPHK1 and SGPL1 mRNA expression levels were detected in HHL-5 cells using reverse transcription-quantitative PCR. (C) SPHK1 and SGPL1 protein expression levels were normalized to actin expression, and p-Akt and p-Erk1/2 protein expression levels were normalized to Akt or Erk1/2 using ImageJ software. *P<0.05, **P<0.01 and ***P<0.001; MCE, medium- and long-chain fat emulsion; Mod, model; p-, phosphorylated; Rec, recovery; SGPL1, sphingosine-1-phosphate lyase 1; SPHK1, sphingosine kinase 1; Rg1, ginsenoside Rg1.