Fig 1: miR-30b-5p targets GRIN2A interfering with the magnesium-free condition that induces the growth inhibition of hippocampal neurons. (a) Gene structure of GRIN2A indicated the predicted target site of miR-30b-5p in its 3'-UTR. (b) Luciferase activity was measured in hippocampal neurons following co-transfecting with WT/MUT GRIN2A 3'-UTR plasmid and miR-30b-5p with the dual luciferase reporter assay. (c) Expression of GRIN2A protein in hippocampal neurons transfected with mimic-NC, miR-30b-5p mimic (mimic), inhibitor-NC, and miR-30b-5p inhibitor (inhibitor) was detected by western blots. (d) Expression of GRIN2A in hippocampal neurons transfected with pcDNA3.1-GRIN2A, pcDNA3.1, miR-30b-5p mimic, and miR-30b-5p mimic + pcDNA3.1-GRIN2A before magnesium-free treatment was investigated by RT-qPCR. (e) Western blotting analysis of GRIN2A expression in hippocampal neurons in the same groups as (d). (f) CCK8 assays determine the cell proliferation in the same groups as (d). (g) Caspase-3 activity measured in the same groups as (d). **P < 0.001. WT, wild type; MUT, mutant.