Fig 2: Knocking out tumor cell IL-6 production significantly reduces M-MDSC and PMN-MDSC recruitment. Tumors and peripheral tissues harvested 28 d after engraftment of 4T1-WT and 4T1-IL6-KO cell lines were analyzed for proportions of (A,B) M-MDSC and (C,D) PMN-MDSC. Healthy, non-tumor-bearing mice were analyzed for comparison (control); see Figure 2 for gating strategy. Data are presented as mean ± SEM; n = 4–11 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Fig 3: Fructose promotes a more inflammatory phenotype.Extracellular cytokine release of A IL-1ß, B IL-6, C IL-8, D IL-10 and E TNF. F Heatmaps displaying the normalised read counts of selected cytokines from RNA-seq analysis of monocytes treated with glucose or fructose (both 11.1 mM) stimulated with LPS (10 ng/mL) for 24 h. G Representative immunoblot and pooled data of downstream mTOR target pS6 with housekeeping control, actin. Statistical significance was assessed using a one-sample t test (A–E) or an unpaired, two-tailed t test (G). Data are representative of five (A, B, D, E), four (C), three (F) and six independent experiments (G) and are expressed as mean ± SEM. Source data are provided as a Source Data file.

Fig 4: Percent of IL-6+ T lymphocytes in blood of mice in each group. (A) Representative flow cytometric analysis. (I)Sorting cell fragments and cells; (II)Sorting cells and T lymphocytes; (III)sorting T lymphocytes and CD3+ T lymphocytes; (IV)Sorting CD3+ T lymphocytes and CD3+ CD4+ T lymphocytes; (V)Sorting CD3+ CD4+ T lymphocytes and IL-6+ CD4+ T lymphocytes. (B) The proportion of CD3+ lymphocytes. (C) The proportion of CD4+ lymphocytes. (D) The proportion of IL-6+ CD4+T lymphocytes. (a) blank control group; (b) repeated restraint stress (RRS) model group; (c) Wendan Decoction group; (d) paroxetine group. *P < 0.05, compared with blank control group; #P < 0.05, compared with RRS model group.

Fig 5: PPI topological analysis of Wendan Decoction treating Generalized Anxiety Disorder. (A)PPI network of 569 targets. (B) The subnet of PPI with 142 hub targets screened based on twice medium degree. (C) According to the Betweenness Centrality, Closeness Centrality, Degree Centrality and Eigenvector Centrality separately, TOP 10 targets in subnet from each criterion were labeled yellow. (D) Venn diagram of 4 topological analysis data. TNF, SRC, IL-6 and AKT1 were common targets.