Fig 2: Sex differences in circulating immune cells in Cohort 2 patients at different days of hospitalization days. A–C Flow cytometric gating strategy for neutrophils, monocytes and lymphocytes in COVID-19 patient peripheral blood. Neutrophils were gated as CD11b+DEspR+ (A), monocytes as CD11b+CD45+CD14+CD24- (B), T cells as CD45+CD3+ and B cells as CD45+CD19+ (C). D Percentage of CD11b+DEspR+ neutrophils in total live leukocytes. E Percentage of CD11b+CD45+CD14+CD24- monocytes in total non-granulocytes. F Percentage of CD45+CD19+ B cells in total lymphocytes. G Percentage of CD45+CD3+ T cells in total lymphocytes. N = 7–26 independent experiments assayed in duplicates. *p < 0.05, **p < 0.01; two-way ANOVA with Sidak’s post hoc correction

Fig 3: FACS analysis for altered expression of CD24 and CD44 after gefitinib treatment. The shaded and closed columns represent the mean percentages of the untreated and treated cells, respectively. The bars represent the SE (n=3). CD24 expressing cells significantly increased during gefitinib treatment, from ~8–23%, with CD44 expressing cells unaltered.

Fig 4: Flow cytometry results of breast cancer stem cells. (A) The average proportion of CD44+/CD24-/lowESA+ MCF7 breast cancer stem cells was 89.4%. (B) The average proportion of CD44+/CD24-/lowESA+ MDA-MB-231 breast cancer stem cells was 57.8%. ESA APC, epithelial surface antigen allophycocyanin; FSC, forward scatter; P1, breast cancer stem cells; P2, CD44+ cells; PE, phycoerythrin; SSC, side scatter.

Fig 5: Single-cell DEPArray™ isolation of uPAR/int ß1 CTC subsets from breast cancer patients.Multiparametric flow cytometry (Six fluorescence channels, ARIA IID system, BD Biosciences™) was applied to select EpCAM-negative/CD45-/CD44+/CD24- CTC followed by DEPArray single-cell isolation to select a combinatorial expression of uPAR (FITC), int ß1 (ApC) and human epidermal growth factor receptor-2 (HER-2) (PE). DEPArray™ (Silicon Biosystems, Inc.) analyses were subsequently performed by Cell BrowserTM software. Representative single CTCs captured and isolated by DEPArray™ are shown. DAPI (ThermoFisher Scientific; cat # D1306) = nuclear staining blue. BF = Brightfield.