Fig 1: Enhancement of Arp2/3-mediated actin polymerization in the presence of ALA. Arp2/3 complex plays an important role in nucleating the actin polymerization and provide pushing force for the cell to move forward. After exposure of Tau monomer, aggregates and ALA the abundance of Arp2/3 in microglia was studied by fluorescence microscopy. a Immunofluorescence images indicate the microglia cells stained with Arp2/3 (red), phalloidin (green), DAPI, scale bar is 20 µm. The enlarged area indicates the colocalization of F-actin and Arp2/3 complex. The enlarged area has been taken from single cell panel and marked with white dotted squares. The white arrow marks indicates concentrated colocalized area inside the cell. b The intracellular intensity of Arp2/3 in the cell was quantified from immunofluorescence images with ZEN 2.3 software and plotted as a fluorescent intensity units (p < 0.05) for ALA treated groups as compared to cell control. c The Pearson’s R value for colocalization was calculated with ImageJ software. The conditions of Tau monomer and aggregtes with ALA are significant, (p < 0.001). d The quantification of protein levels of Arp2/3 was observed from western blot analysis after 24 h treatment of Tau monomer, aggregates and ALA. e The graph indicates quantification of western blot bands normalized with the loading control ß-tubulin. The conditions of Tau monomer and aggregtes with ALA are significant, (p < 0.05). The significance was analyzed with Tukey’s Kramer, significant when mean difference between treatment groups (X-X’) > T (Tukey’s criteria). The annotations used in graph are as follows- CC (Cell control), ALA (a-linolenic acid), Mono. (monomer), Agg. (aggregates), Mono. + ALA (monomer plus ALA), Agg. + ALA (aggregates plus ALA)