Fig 1: NFZ targets the ETS domain of ERG. A, ERG, ALDH1, and STAT3 Western blots. Samples are untreated lysates from five different cell lines. Uncropped blots are shown in Supplementary Fig. S2. B, The quantification of ERG, ALDH1, and STAT3 was normalized to total protein and analyzed with Image Lab 5.2 software using the first sample as a reference. C, ERG and NFZ affinity determination curve. NFZ concentrations decrease from top to bottom. D, The fitted curve of the affinity. E, Expression of genes downstream of ERG, determined by RT-qPCR. RQ, relative quantification. DMSO was used as a control. The abscissa indicates 2 or 5 µmol/L NFZ treatment for 12 or 24 hours. Results are shown as mean ± SD (n = 3 independent experiments; ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001), compared with the DMSO control, by Dunnett multiple comparisons test.